Discovery of a Redox Thiol Switch: Implications for Cellular Energy Metabolism.

Molecular & Cellular Proteomics : MCP
Xing-Huang GaoMaria Hatzoglou

Abstract

The redox-based modifications of cysteine residues in proteins regulate their function in many biological processes. The gas molecule H2S has been shown to persulfidate redox sensitive cysteine residues resulting in an H2S-modified proteome known as the sulfhydrome. Tandem Mass Tags (TMT) multiplexing strategies for large-scale proteomic analyses have become increasingly prevalent in detecting cysteine modifications. Here we developed a TMT-based proteomics approach for selectively trapping and tagging cysteine persulfides in the cellular proteomes. We revealed the natural protein sulfhydrome of two human cell lines, and identified insulin as a novel substrate in pancreatic beta cells. Moreover, we showed that under oxidative stress conditions, increased H2S can target enzymes involved in energy metabolism by switching specific cysteine modifications to persulfides. Specifically, we discovered a Redox Thiol Switch, from protein S-glutathioinylation to S-persulfidation (RTSGS). We propose that the RTSGS from S-glutathioinylation to S-persulfidation is a potential mechanism to fine tune cellular energy metabolism in response to different levels of oxidative stress.

References

Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maddalena FratelliPietro Ghezzi
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dana L Miller, Mark B Roth
Sep 9, 2008·Antioxidants & Redox Signaling·John J MieyalMelissa D Shelton
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosa E HansenJakob R Winther
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
Nov 12, 2009·Science Signaling·Asif K MustafaSolomon H Snyder
May 8, 2010·The Journal of Biological Chemistry·Omer Kabil, Ruma Banerjee
Aug 21, 2010·Trends in Endocrinology and Metabolism : TEM·Ming LiuPeter Arvan
Jul 26, 2011·Plant Physiology and Biochemistry : PPB·Karina van der LindeRenate Scheibe
Nov 30, 2011·Molecular & Cellular Proteomics : MCP·Mirko ZaffagniniStéphane D Lemaire
Jul 12, 2012·Nature Reviews. Molecular Cell Biology·Bindu D Paul, Solomon H Snyder
Jul 25, 2012·Trends in Endocrinology and Metabolism : TEM·Weibo Luo, Gregg L Semenza
Aug 14, 2012·Analytical Chemistry·Graeme C McAlisterSteven P Gygi
Dec 5, 2012·Nature Reviews. Genetics·A F Maarten AltelaarAlbert J R Heck
Dec 12, 2012·The Journal of Biological Chemistry·Leena HaatajaPeter Arvan
Jul 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Csaba SzaboMark R Hellmich
Jan 24, 2014·Redox Biology·Ryan J MaillouxWilliam G Willmore
Jul 31, 2014·Nature Methods·Neville E SanjanaFeng Zhang
Jun 18, 2015·Nature Chemical Biology·Tatiana V MishaninaRuma Banerjee
Oct 7, 2015·Trends in Biochemical Sciences·Bindu D Paul, Solomon H Snyder
Feb 26, 2016·Molecular Metabolism·Marion BenazraPhilippe Ravassard
May 27, 2016·Antioxidants & Redox Signaling·Philipp A Gerber, Guy A Rutter
May 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Alexander MironovEvgeny Nudler
Nov 8, 2017·Chemical Reviews·Milos R FilipovicRuma Banerjee

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Citations

Jun 17, 2020·Molecular & Cellular Proteomics : MCP·Steven T BruckbauerMichael R Sussman
Jun 17, 2020·Frontiers in Genetics·Hongchun ZhaoQingyin Zheng
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Steven T BruckbauerMichael R Sussman

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