Discovery of Calcium as a Biofilm-Promoting Signal for Vibrio fischeri Reveals New Phenotypes and Underlying Regulatory Complexity

Journal of Bacteriology
Alice H TischlerKaren L Visick

Abstract

Vibrio fischeri uses biofilm formation to promote symbiotic colonization of its squid host, Euprymna scolopes Control over biofilm formation is exerted at the level of transcription of the symbiosis polysaccharide (syp) locus by a complex set of two-component regulators. Biofilm formation can be induced by overproduction of the sensor kinase RscS, which requires the activities of the hybrid sensor kinase SypF and the response regulator SypG and is negatively regulated by the sensor kinase BinK. Here, we identify calcium as a signal that promotes biofilm formation by biofilm-competent strains under conditions in which biofilms are not typically observed (growth with shaking). This was true for RscS-overproducing cells as well as for strains in which only the negative regulator binK was deleted. The latter results provided, for the first time, an opportunity to induce and evaluate biofilm formation without regulator overexpression. Using these conditions, we determined that calcium induces both syp-dependent and bacterial cellulose synthesis (bcs)-dependent biofilms at the level of transcription of these loci. The calcium-induced biofilms were dependent on SypF, but SypF's Hpt domain was sufficient for biofilm formation. These da...Continue Reading

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Citations

May 20, 2018·Applied and Environmental Microbiology·Karen L VisickVincent Mastrodomenico
Sep 19, 2018·Journal of Bacteriology·Itai MuzhingiAlexandra E Purdy
Oct 10, 2018·Molecular Microbiology·Cecilia M ThompsonKaren L Visick
Feb 21, 2019·Journal of Bacteriology·Ella R RotmanMark J Mandel
Jun 5, 2020·Current Protocols in Microbiology·David G Christensen, Karen L Visick
Jul 22, 2018·Applied and Environmental Microbiology·Cecilia M ThompsonKaren L Visick
Sep 18, 2020·BMC Microbiology·Elena Laura SteigerMonika Astasov-Frauenhoffer
Oct 9, 2019·Current Opinion in Microbiology·Clotilde Bongrand, Edward G Ruby
Nov 15, 2020·Applied and Environmental Microbiology·Pat M FidopiastisKaren L Visick
Jan 6, 2021·Applied and Environmental Microbiology·Joshua J CohenKaren L Visick
Mar 11, 2021·MBio·Katherine E ZinkLaura M Sanchez
Jun 6, 2021·Nature Reviews. Microbiology·Karen L VisickEdward G Ruby
May 1, 2021·Journal of Bacteriology·Ákos T Kovács, Nicola R Stanley-Wall
Dec 24, 2021·Bioscience Reports·Cameron Lee-Lopez, Erik Yukl

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