Discovery of direct-acting antiviral agents with a graphene-based fluorescent nanosensor.

Science Advances
Se-Jin ParkDal-Hee Min

Abstract

Direct-acting agents against viral components are considered as the most promising candidates for the successful antiviral therapeutics. To date, no direct-acting drugs exist for the treatment against dengue virus (DV) infection, which can develop into life-threatening diseases. RNA-dependent RNA polymerase (RdRp), an RNA virus-specific enzyme highly conserved among various viral families, has been known as the broad-range antiviral drug target. Here, we developed an RNA-based graphene biosensor system [RNA nano-graphene oxide system (RANGO)] to enable the fluorescence-based quantitative analysis of the RdRp enzyme activity. We used the RANGO system to a high-throughput chemical screening to identify novel direct-acting antiviral drug candidates targeting DV RdRp from the FDA-approved small-molecule library. RANGO accelerated the massive selection of drug candidates. We found that one of the selected hit compounds, montelukast, showed antiviral activity in vitro and in vivo by directly inhibiting replication of DV and thus relieved related symptoms.

References

Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Aug 1, 1952·Proceedings of the National Academy of Sciences of the United States of America·R Dulbecco
May 12, 2007·The Journal of Infectious Diseases·Jennifer L KyleEva Harris
May 29, 2009·Angewandte Chemie·Chun-Hua LuGuo-Nan Chen
Sep 16, 2009·Annals of the New York Academy of Sciences·Katherine L WilliamsEva Harris
Oct 2, 2009·Antiviral Chemistry & Chemotherapy·Ye ChenNeerja Kaushik-Basu
Oct 14, 2009·Clinical Microbiology Reviews·Byron E E MartinaAlbert D M E Osterhaus
Feb 16, 2010·Antiviral Research·Min QingPei Yong Shi
Aug 14, 2010·Advanced Materials·Yanwu ZhuRodney S Ruoff
Jul 19, 2012·The Journal of Infectious Diseases·Nguyet Minh NguyenCameron P Simmons
Feb 14, 2013·Antimicrobial Agents and Chemotherapy·Chelsea M ByrdRobert Jordan
Mar 14, 2013·Molecules and Cells·Hongje JangDal-Hee Min
Jun 2, 2015·Cell·Tuan Anh NguyenJae-Sung Woo
Jun 10, 2016·Clinical Microbiology Reviews·Erik De Clercq, Guangdi Li
Jan 21, 2017·Analytical Chemistry·Jungho KimDal-Hee Min
Apr 14, 2017·The Journal of Infectious Diseases·Jenny G H LowSubhash G Vasudevan
Aug 24, 2018·Open Biology·Abhay P S Rathore, Ashley L St John
Nov 19, 2019·PLoS Neglected Tropical Diseases·Hideaki ShimizuTatsuo Shioda

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
biosensors
atomic force microscopy
RNA assay
transgenic
biosensor
transfection
dynamic light scattering

Software Mentioned

R (
RANGO
OriginPro
R Foundation for Statistical Computing

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Antiviral Therapy
Alexander Thompson, Stephen Locarnini
Journal of Clinical and Experimental Hepatology
Madhumita Premkumar, Radha K Dhiman
The Lancet Infectious Diseases
Stelliana GoutzamanisPeter Higgs
© 2022 Meta ULC. All rights reserved