Discovery of Four Novel Viruses Associated with Flower Yellowing Disease of Green Sichuan Pepper (Zanthoxylum Armatum ) by Virome Analysis

Viruses
Mengji CaoYan Zhou

Abstract

An emerging virus-like flower yellowing disease (FYD) of green Sichuan pepper (Zanthoxylum armatum v. novemfolius) has been recently reported. Four new RNA viruses were discovered in the FYD-affected plant by the virome analysis using high-throughput sequencing of transcriptome and small RNAs. The complete genomes were determined, and based on the sequence and phylogenetic analysis, they are considered to be new members of the genera Nepovirus (Secoviridae), Idaeovirus (unassigned), Enamovirus (Luteoviridae), and Nucleorhabdovirus (Rhabdoviridae), respectively. Therefore, the tentative names corresponding to these viruses are green Sichuan pepper-nepovirus (GSPNeV), -idaeovirus (GSPIV), -enamovirus (GSPEV), and -nucleorhabdovirus (GSPNuV). The viral population analysis showed that GSPNeV and GSPIV were dominant in the virome. The small RNA profiles of these viruses are in accordance with the typical virus-plant interaction model for Arabidopsis thaliana. Rapid and sensitive RT-PCR assays were developed for viral detection, and used to access the geographical distributions. The results revealed a correlation between GSPNeV and the FYD. The viruses pose potential threats to the normal production of green Sichuan pepper in the aff...Continue Reading

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Citations

Jul 8, 2020·Archives of Virology·Zeli ChenMingfu Zhao
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Nai-Tong YuZhi-Xin Liu
Oct 13, 2020·Frontiers in Microbiology·Nicolás BejermanRalf G Dietzgen
Apr 7, 2021·Virus Research·Kahraman GürcanKadriye Çağlayan

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Datasets Mentioned

BETA
MH323435
MH323434
MH323432
MH323433
MH323437
MH323436

Methods Mentioned

BETA
sRNA-seq
RNA-seq
PCR
chips

Software Mentioned

Velvet
Trinity
BLASTp Genomics
MUSCLE
BLASTn
CLC Genomics Workbench
BLASTx
Mega

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