Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein.

BioRxiv : the Preprint Server for Biology
P. Heinzelman, Philip A Romero


Understanding how human ACE2 genetic variants differ in their recognition by SARS-CoV-2 can have a major impact in leveraging ACE2 as an axis for treating and preventing COVID-19. In this work, we experimentally interrogate thousands of ACE2 mutants to identify over one hundred human single-nucleotide variants (SNVs) that are likely to have altered recognition by the virus, and make the complementary discovery that ACE2 residues distant from the spike interface can have a strong influence upon the ACE2-spike interaction. These findings illuminate new links between ACE2 sequence and spike recognition, and will find wide-ranging utility in SARS-CoV-2 fundamental research, epidemiological analyses, and clinical trial design.


Apr 27, 2021·Frontiers in Molecular Biosciences·Krishna K Narayanan, Erik Procko
Jun 3, 2021·Viruses·Thomas D Burton, Nicholas S Eyre
Jun 8, 2021·Frontiers in Veterinary Science·Herbert F JelinekHabiba S Alsafar

Methods Mentioned

fluorescence activated cell sorting
Illumina sequencing
flow cytometry

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.