Mar 31, 2020

Two new coral fluorescent proteins of distinct colorsfor sharp visualization of cell-cycle progression

BioRxiv : the Preprint Server for Biology
Marzieh Eslami RasekhCan Alkan

Abstract

We cloned and characterized two new coral fluorescent proteins: h2-3 and 1-41. h2-3 formed an obligate dimeric complex and exhibited bright fluorescence. On the other hand, 1-41 formed a highly multimeric complex and exhibited dim red fluorescence. We engineered 1-41 into AzaleaB5, a practically useful red-emitting fluorescent protein for cellular labeling applications. We fused h2-3 and AzaleaB5 to the ubiquitination domains of human Geminin and Cdt1, respectively, to generate a new color variant of Fucci (Fluorescent Ubiquitination-based Cell-Cycle Indicator): Fucci5. We found Fucci5 provided brighter nuclear labeling for monitoring cell cycle progression than the 1st and 2nd generations that used mAG/mKO2 and mVenus/mCherry, respectively.

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Mentioned in this Paper

Fluorescent in Situ Hybridization
Genome
Segmental Duplications, Genomic
Inversion Mutation Abnormality
Genome Assembly Sequence
Nucleic Acid Sequencing
Nesara francesca
Clone
Genomics
Sequencing

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