Discovery of microRNA-like RNAs during early fruiting body development in the model mushroom Coprinopsis cinerea

PloS One
Amy Yuet Ting LauHoi Shan Kwan

Abstract

Coprinopsis cinerea is a model mushroom particularly suited for the study of fungal fruiting body development and the evolution of multicellularity in fungi. While microRNAs (miRNAs) have been extensively studied in animals and plants for their essential roles in post-transcriptional regulation of gene expression, miRNAs in fungi are less well characterized and their potential roles in controlling mushroom development remain unknown. To identify miRNA-like RNAs (milRNAs) in C. cinerea and explore their expression patterns during the early developmental transition of mushroom development, small RNA libraries of vegetative mycelium and primordium were generated and putative milRNA candidates were identified following the standards of miRNA prediction in animals and plants. Two out of 22 novel predicted milRNAs, cci-milR-12c and cci-milR-13e-5p, were validated by northern blot and stem-loop reverse transcription real-time PCR. Cci-milR-12c was differentially expressed whereas the expression levels of cci-milR-13e-5p were similar in the two developmental stages. Target prediction of the validated milRNAs resulted in genes associated with fruiting body development, including pheromone, hydrophobin, cytochrome P450, and protein kinas...Continue Reading

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Citations

Mar 20, 2020·Molecular Biology and Evolution·Zsolt MerényiLászló G Nagy
Dec 12, 2020·International Journal of Molecular Sciences·Carlos LaxSilvia Calo

Datasets Mentioned

BETA
AACS00000000
RF02543

Methods Mentioned

BETA
PCR
transfection
gene knockouts

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