Discovery of Novel Cell Surface Markers for Purification of Embryonic Dopamine Progenitors for Transplantation in Parkinson's Disease Animal Models.

Molecular & Cellular Proteomics : MCP
Ali FathiGhasem Hosseini Salekdeh

Abstract

Despite the progress in safety and efficacy of cell replacement therapy with pluripotent stem cells (PSCs), the presence of residual undifferentiated stem cells or proliferating neural progenitor cells with rostral identity remains a major challenge. Here we report the generation of a LIM homeobox transcription factor 1 alpha (LMX1A) knock-in GFP reporter human embryonic stem cell (hESC) line that marks the early dopaminergic progenitors during neural differentiation to find reliable membrane protein markers for isolation of midbrain dopaminergic neurons. Purified GFP positive cells in vitro exhibited expression of mRNA and proteins that characterized and matched the midbrain dopaminergic identity. Further quantitative proteomics analysis of enriched LMX1A+ cells identified several membrane-associated proteins including a polysialylated embryonic form of neural cell adhesion molecule (PSA-NCAM) and contactin 2 (CNTN2), enabling prospective isolation of LMX1A+ progenitor cells. Transplantation of human-PSC-derived purified CNTN2+ progenitors enhanced dopamine release from transplanted cells in the host brain and alleviated Parkinson's disease-related phenotypes in animal models. This study establishes an efficient approach for p...Continue Reading

References

Mar 10, 2001·The New England Journal of Medicine·C R FreedS Fahn
Sep 4, 2003·Annals of Neurology·C Warren OlanowThomas B Freeman
Jul 24, 2004·Differentiation; Research in Biological Diversity·Hossein BaharvandDavood Sabour
Jun 1, 2005·Behavioural Brain Research·Ruxandra IancuGesine Paul
Jan 28, 2006·Cell·Elisabet AnderssonJohan Ericson
Jul 11, 2006·Journal of Proteome Research·Jeroen KrijgsveldAlbert J R Heck
Apr 21, 2007·Nature Protocols·Magdaline CostaEdouard G Stanley
Apr 25, 2007·Brain : a Journal of Neurology·Manolo CartaAnders Björklund
Jul 27, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thomas CarlssonDeniz Kirik
Jan 9, 2009·Bioinformatics·Uma MudunuriRobert M Stephens
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stina FrilingJohan Ericson
Jun 3, 2009·Antioxidants & Redox Signaling·Kathleen M FitzpatrickMarina E Emborg
Jul 18, 2009·Neuron·Lujian LiaoJohn R Yates
Jan 12, 2010·Methods : a Companion to Methods in Enzymology·Barbara D'haeneJan Hellemans
May 14, 2010·Brain Research·Sudarshan PhaniLorraine Iacovitti
Jul 2, 2010·Science Translational Medicine·Marios PolitisPaola Piccini
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sangmi ChungKwang-Soo Kim
Jul 4, 2012·The Journal of Clinical Investigation·Yosif M GanatLorenz Studer
Dec 13, 2012·Parkinsonism & Related Disorders·Sruti RayaproluOwen A Ross
Nov 26, 2013·Journal of Proteome Research·Mehdi MirzaeiGhasem Hosseini Salekdeh
Sep 27, 2014·World Journal of Stem Cells·Tetsuya Ishii, Koji Eto
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chris R ByeLachlan H Thompson

❮ Previous
Next ❯

Citations

Oct 24, 2018·Proteomics·David C HandlerPaul A Haynes
Oct 26, 2018·Expert Review of Proteomics·Faezeh ShekariGhasem Hosseini Salekdeh
Jan 9, 2020·Translational Neurodegeneration·Yong FanShi-Yan Ng
Jul 28, 2020·Cellular and Molecular Life Sciences : CMLS·Anna MeyfourGhasem Hosseini Salekdeh
Dec 7, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liting DengPaul A Haynes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.