Discovery of Novel Leptospirosis Vaccine Candidates Using Reverse and Structural Vaccinology

Frontiers in Immunology
André A GrassmannAlan J A McBride

Abstract

Leptospira spp. are diderm (two membranes) bacteria that infect mammals causing leptospirosis, a public health problem with global implications. Thousands of people die every year due to leptospirosis, especially in developing countries with tropical climates. Prophylaxis is difficult due to multiple factors, including the large number of asymptomatic hosts that transmit the bacteria, poor sanitation, increasing numbers of slum dwellers, and the lack of an effective vaccine. Several leptospiral recombinant antigens were evaluated as a replacement for the inactivated (bacterin) vaccine; however, success has been limited. A prospective vaccine candidate is likely to be a surface-related protein that can stimulate the host immune response to clear leptospires from blood and organs. In this study, a comprehensive bioinformatics approach based on reverse and structural vaccinology was applied toward the discovery of novel leptospiral vaccine candidates. The Leptospira interrogans serovar Copenhageni strain L1-130 genome was mined in silico for the enhanced identification of conserved β-barrel (βb) transmembrane proteins and outer membrane (OM) lipoproteins. Orthologs of the prospective vaccine candidates were screened in the genomes...Continue Reading

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Nov 30, 2019·Expert Opinion on Drug Discovery·Carolina R FelixAlan J A McBride
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Datasets Mentioned

BETA
AE016823.1
AE016824.1

Methods Mentioned

BETA
X-ray

Software Mentioned

PyMOL
CoVIRA
BOMP
Qmean6
NetMHCII
Python
MEMSAT
QMEAN
SignalCF
ModFOLD4

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