Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing: Mafa-class I polymorphism

Immunogenetics
Takashi ShiinaAntoine Blancher

Abstract

Although the low polymorphism of the major histocompatibility complex (MHC) transplantation genes in the Filipino cynomolgus macaque (Macaca fascicularis) is expected to have important implications in the selection and breeding of animals for medical research, detailed polymorphism information is still lacking for many of the duplicated class I genes. To better elucidate the degree and types of MHC polymorphisms and haplotypes in the Filipino macaque population, we genotyped 127 unrelated animals by the Sanger sequencing method and high-resolution pyrosequencing and identified 112 different alleles, 28 at cynomolgus macaque MHC (Mafa)-A, 54 at Mafa-B, 12 at Mafa-I, 11 at Mafa-E, and seven at Mafa-F alleles, of which 56 were newly described. Of them, the newly discovered Mafa-A8*01:01 lineage allele had low nucleotide similarities (<86%) with primate MHC class I genes, and it was also conserved in the Vietnamese and Indonesian populations. In addition, haplotype estimations revealed 17 Mafa-A, 23 Mafa-B, and 12 Mafa-E haplotypes integrated with 84 Mafa-class I haplotypes and Mafa-F alleles. Of these, the two Mafa-class I haplotypes, F/A/E/B-Hp1 and F/A/E/B-Hp2, had the highest haplotype frequencies at 10.6 and 10.2%, respectivel...Continue Reading

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Citations

Oct 13, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natasja G de GrootRonald E Bontrop
Feb 27, 2018·Immunogenetics·Marit K H van der WielR E Bontrop
Apr 2, 2019·Investigative Ophthalmology & Visual Science·Kenichi MakabeMasayo Takahashi
Oct 5, 2019·Journal of Clinical Medicine·Iori KisuTakashi Shiina
May 3, 2020·International Journal of Immunogenetics·Corrine M C HeijmansRonald E Bontrop
Jul 19, 2020·Primates; Journal of Primatology·Lief Erikson GamaloAndie Ang
Nov 28, 2018·Inflammation and Regeneration·Hirohito IshigakiKazumasa Ogasawara
Sep 1, 2017·Nature Communications·Asuka MorizaneJun Takahashi
Aug 29, 2019·Cells·Takashi Shiina, Antoine Blancher
Nov 21, 2020·Journal of Clinical Medicine·Iori KisuTakashi Shiina
Apr 4, 2021·International Journal of Molecular Sciences·Masaaki IshidaMasayo Takahashi
Jan 31, 2019·Journal of Proteome Research·Yanjuan LiQuan Zou

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