Discovery of novel rhabdoviruses in the blood of healthy individuals from West Africa

PLoS Neglected Tropical Diseases
Matthew H StremlauChristian T Happi

Abstract

Next-generation sequencing (NGS) has the potential to transform the discovery of viruses causing unexplained acute febrile illness (UAFI) because it does not depend on culturing the pathogen or a priori knowledge of the pathogen's nucleic acid sequence. More generally, it has the potential to elucidate the complete human virome, including viruses that cause no overt symptoms of disease, but may have unrecognized immunological or developmental consequences. We have used NGS to identify RNA viruses in the blood of 195 patients with UAFI and compared them with those found in 328 apparently healthy (i.e., no overt signs of illness) control individuals, all from communities in southeastern Nigeria. Among UAFI patients, we identified the presence of nucleic acids from several well-characterized pathogenic viruses, such as HIV-1, hepatitis, and Lassa virus. In our cohort of healthy individuals, however, we detected the nucleic acids of two novel rhabdoviruses. These viruses, which we call Ekpoma virus-1 (EKV-1) and Ekpoma virus-2 (EKV-2), are highly divergent, with little identity to each other or other known viruses. The most closely related rhabdoviruses are members of the genus Tibrovirus and Bas-Congo virus (BASV), which was recen...Continue Reading

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Citations

Oct 23, 2019·The Journal of Applied Laboratory Medicine·Alexander L Greninger, Samia N Naccache
Feb 29, 2020·Viruses·Jens H KuhnMatthew Stremlau
Mar 23, 2017·PLoS Pathogens·Ahmed MoustafaAmalio Telenti
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Mar 27, 2021·Scientific Reports·María Cebriá-MendozaJosé M Cuevas

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Datasets Mentioned

BETA
PRJNA271229
KP324827
KP324828

Methods Mentioned

BETA
reverse transcription PCR
PCR
ELISA
Illumina sequencing
enzyme-linked immunosorbent assays
blood draw
electron microscopy
ELISAs
RNA-seq

Software Mentioned

RAxML
Trinity
PRINSEQ
MAFFT
BMTagger
BLASTn
MetaVelvet
PROTTEST
BLASTx
trimAl

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