Discovery of Pantoea stewartii ssp. stewartii genes important for survival in corn xylem through a Tn-Seq analysis

Molecular Plant Pathology
Duy An DuongAnn M Stevens

Abstract

The bacterium Pantoea stewartii ssp. stewartii causes Stewart's wilt disease in corn. Pantoea stewartii is transmitted to plants via corn flea beetles, where it first colonizes the apoplast causing water-soaked lesions, and then migrates to the xylem and forms a biofilm that blocks water transport. Bacterial quorum sensing ensures that the exopolysaccharide production necessary for biofilm formation occurs only at high cell density. A genomic-level transposon sequencing (Tn-Seq) analysis was performed to identify additional bacterial genes essential for survival in planta and to provide insights into the plant-microbe interactions occurring during wilt disease. A mariner transposon library of approximately 40 000 mutants was constructed and used to inoculate corn seedlings through a xylem infection model. Cultures of the library grown in Luria-Bertani (LB) broth served as the in vitro pre-inoculation control. Tn-Seq analysis showed that the number of transposon mutations was reduced by more than 10-fold for 486 genes in planta compared with the library that grew in LB, suggesting that they are important for xylem survival. Interestingly, a small set of genes had a higher abundance of mutants in planta versus in vitro conditions...Continue Reading

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Citations

Sep 30, 2018·Molecular Plant Pathology·Kévin RoyetGuy Condemine
Sep 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tyler C HelmannSteven E Lindow
Apr 8, 2020·Frontiers in Plant Science·Belinda K FabianIan T Paulsen
Jun 14, 2020·Nature Reviews. Genetics·Amy K CainTim van Opijnen
Nov 24, 2020·Phytopathology·Yuan Zeng, Amy O Charkowski
Mar 1, 2019·G3 : Genes - Genomes - Genetics·Ramamoorthy SivakumarJeyaprakash Rajendhran

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