Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies.

Analytical Chemistry
Jixing LyuArthur Laganowsky

Abstract

There is growing interest in the characterization of protein complexes and their interactions with ligands using native ion mobility mass spectrometry. A particular challenge, especially for membrane proteins, is preserving noncovalent interactions and maintaining native-like structures. Different approaches have been developed to minimize activation of protein complexes by manipulating charge on protein complexes in solution and the gas-phase. Here, we report the utility of polyamines that have exceptionally high charge-reducing potencies with some molecules requiring 5-fold less than trimethylamine oxide to elicit the same effect. The charge-reducing molecules do not adduct to membrane protein complexes and are also compatible with ion-mobility mass spectrometry, paving the way for improved methods of charge reduction.

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Citations

Sep 30, 2020·Nature Chemical Biology·Samantha SchreckeArthur Laganowsky
Mar 19, 2021·Journal of the American Society for Mass Spectrometry·Christian IeritanoW Scott Hopkins
Apr 23, 2021·Journal of the American Society for Mass Spectrometry·Iain D G Campuzano, Wendy Sandoval
Oct 30, 2020·Analytical Chemistry·James E KeenerMichael T Marty
Aug 28, 2021·Chemical Reviews·Jack L BennettWilliam A Donald
Sep 3, 2021·Chemical Reviews·Amber D Rolland, James S Prell

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