Discovery of restless legs syndrome plasmatic biomarkers by proteomic analysis

Brain and Behavior
Elisa BelleiStefania Bergamini

Abstract

Restless legs syndrome (RLS) can lead to severe clinical consequences, thus negatively impacts on patients' overall health and quality of life. Nevertheless, the pathophysiology of RLS is still unclear, resulting in underestimate, incorrect, or ignored diagnosis and in limited management and treatment. The aim of this study was to compare the plasma proteome of RLS patients and healthy controls, in the search of diagnostic biomarkers related to the disease severity. Two-dimensional gel electrophoresis coupled with liquid chromatography-mass spectrometry was employed to analyze plasma samples of 34 patients with primary RLS, divided into two subgroups according to the disease severity: MMS group (mild-moderate symptoms) and HS group (severe and very severe symptoms), and 17 age- and sex-matched control subjects. Sleep quality, daytime sleepiness, and the level of depression were also evaluated. We identified eight upregulated spots, corresponding to five unique proteins, in both RLS group vs. controls (alpha-1B-glycoprotein, alpha-1-acid glycoprotein 1, haptoglobin, complement C4-A, and immunoglobulin kappa constant); five increased spots, consistent with three unique proteins, only in HS-RLS (kininogen-1, immunoglobulin heavy c...Continue Reading

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Citations

Jan 30, 2020·Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine·Leonard B WeinstockGerhard J Molderings
Aug 4, 2020·Frontiers in Neuroscience·Adrian Tudor BalseanuAurel Popa-Wagner
Jul 25, 2019·Journal of Neural Transmission·Elisa BelleiSerkan Ozben
Apr 24, 2021·Clinical Psychopharmacology and Neuroscience : the Official Scientific Journal of the Korean College of Neuropsychopharmacology·Cem CeritGürler Akpınar
Jul 9, 2021·Nature and Science of Sleep·Stefania MondelloRaffaele Ferri
Jul 1, 2021·Cardiology and Therapy·Lyudmila Korostovtseva
Jun 3, 2020·Journal of Proteome Research·Xue DongYehia Mechref

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Methods Mentioned

BETA
electrophoresis
immunodepletion
ELISA

Software Mentioned

MASCOT
PDQuest

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