Discrepancy between cytokine production from peripheral blood mononuclear cells and nasal secretions among infants with acute bronchiolitis

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
Paulo Márcio Condessa PitrezRenato Tetelbom Stein

Abstract

Many studies have measured cytokine production derived from peripheral blood mononuclear cells (PBMCs) to evaluate the immune response in acute bronchiolitis (AB), but no previous reports have examined the association between PBMC release of cytokines and concomitant airway immune response. To determine whether interferon-gamma (IFN-gamma), interleukin 4 (IL-4), and IL-10 levels from PBMCs are associated with concurrent cytokine release in the airways of infants with AB. Infants with acute viral-associated first episode of wheezing who required hospitalization between May and September 2002 were recruited. Nasopharyngeal aspirates (NPAs) and PBMC samples were collected simultaneously. The concentrations of IFN-gamma, IL-4, and IL-10 in NPA and PBMC supernatants were determined by enzyme-linked immunosorbent assay. Twenty infants with AB were enrolled in the study of whom 17 (85%) had positive NPA immunofluorescence results for viral detection and respiratory syncytial virus. Median total cell count and viability from NPA samples were 2.2 x 10(6) cells/mL (SD, 1.7 cells/mL) and 92% (SD, 6.0%), respectively. There was a significant correlation between IL-4 levels from NPA and PBMC samples (r = 0.5, P = .02); however, we did not f...Continue Reading

References

Oct 1, 1995·The Journal of Pediatrics·J M IngramP W Heymann
Nov 1, 1994·Archives of Disease in Childhood·M L EverardA D Milner
Jul 1, 1997·American Journal of Respiratory and Critical Care Medicine·M RománP V Díaz
Feb 27, 1999·The Pediatric Infectious Disease Journal·G YilmazS Badur
Apr 13, 1999·The Journal of Allergy and Clinical Immunology·S M van SchaikR C Welliver
Oct 6, 1999·American Journal of Respiratory and Critical Care Medicine·P V DíazA Díaz
Aug 2, 2000·Pediatric Pulmonology·S M van SchaikJ L Kimpen
May 10, 2002·Pediatric Pulmonology·Kevin J MobbsC Anthony Hart

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Citations

Sep 30, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G C MüllerM E Bauer
Jul 25, 2006·Lancet·Rosalind L Smyth, Peter J M Openshaw
Apr 27, 2007·Allergology International : Official Journal of the Japanese Society of Allergology·Hiroki MuraiHajime Togari
May 21, 2009·Expert Opinion on Emerging Drugs·Wieslawa Olszewska, Peter Openshaw

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