Discrepancy between latex and ELISA D-dimer values in sepsis may be caused by human neutrophil elastase

Thrombosis Research
E T Bach-GansmoO H Skjønsberg

Abstract

We have recently shown that D-dimers are degraded by human neutrophil elastase (HNE) in vitro, causing rapid decrease in the D-dimer levels measured by a Latex test, but not with an ELISA test employing the same monoclonal antibody against D-dimer. To see if such discrepant D-dimer concentrations occurred in patients with high HNE concentration, we examined 80 plasma samples from 8 patients with sepsis with a Latex and an ELISA test and calculated the ratio between the D-dimer values obtained with the two tests. Twenty healthy pregnant and twenty pre-eclamptic patients, who are known to have raised D-dimer but low HNE concentrations, were chosen as controls. HNE levels were estimated by determining the HNE-alpha 1-proteinase inhibitor complex (HNE-A1PI) concentration. HNE-A1PI concentration was increased in sepsis patients compared with pre-eclamptic patients (p < 0.0005) and healthy pregnant women (p < 0.0005). In sepsis patients, the D-dimer results were skewed towards lower ratios between Latex and ELISA values compared to pre-eclamptic patients (p = 0.008) and healthy pregnant women (p = 0.0001). In plasma samples from patients with the largest discrepancy between Latex and ELISA D-dimer values, Western blotting with immuno...Continue Reading

References

Aug 1, 1989·British Journal of Obstetrics and Gynaecology·I A GreerA A Calder
Jun 1, 1986·The Journal of Pediatrics·C P SpeerM Gahr
Jan 1, 1985·Annual Review of Medicine·A Janoff
Dec 30, 1972·Lancet·P J Gaffney
Dec 1, 1994·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·K KolevR Machovich
Apr 15, 1996·Thrombosis Research·E T Bach-GansmoO H Skjonsberg
Sep 1, 2005·The Journal of Laboratory and Clinical Medicine·Georg AueMcDonald K Horne

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Citations

Apr 11, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·N FiottiC Giansante
Jan 27, 2005·Journal of Thrombosis and Haemostasis : JTH·C-E Dempfle
Jun 26, 2009·American Journal of Hematology·Fred J L M HaasDouwe H Biesma

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