Discrepancy between mRNA and protein expression of tumour suppressor maspin in synovial tissue may contribute to synovial hyperplasia in rheumatoid arthritis

Annals of the Rheumatic Diseases
J SchedelS Gay

Abstract

To investigate the expression of maspin in RA synovial tissue and compare it with the expression in osteoarthritis (OA) and normal synovial tissue (NS). Using specific primers for maspin, a 237 bp fragment was amplified from cDNA obtained from cultured RA, OA, and normal synovial fibroblasts (SF) by RT-PCR. Additionally, mRNA expression levels were determined quantitatively by real time PCR. mRNA expression of maspin was investigated on snap frozen and paraffin embedded synovial tissue sections by in situ hybridisation. Immunohistochemistry was used to identify the cell type expressing maspin. SDS-PAGE and western blotting were performed to evaluate the protein expression in cultured SF. To confirm protein synthesis in situ, immunohistochemistry with specific anti-maspin antibodies was performed in synovial tissue sections of patients with RA. RT-PCR showed expression of maspin in all cDNA samples from cultured SF. Maspin mRNA was found to be decreased in RA SF twofold and 70-fold compared with OA SF and NS SF, respectively. Maspin mRNA was expressed in RA, OA, and normal synovial tissue. Importantly, maspin transcripts were also found at sites of invasion into cartilage and bone. At the protein level, maspin could be detected ...Continue Reading

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Citations

Aug 26, 2006·Nature Clinical Practice. Rheumatology·Ulf Müller-LadnerSteffen Gay
Aug 7, 2012·Tissue Engineering. Part a·Marijn RutgersLaura B Creemers
Dec 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zhila Khalkhali-Ellis
Apr 28, 2006·Investigative Ophthalmology & Visual Science·Louis TongStephen C Pflugfelder
Jan 16, 2008·BMC Molecular Biology·Michel NoutsiasKatja Kotsch
Mar 18, 2006·The Journal of Physiology·William J Brackenbury, Mustafa B A Djamgoz
Dec 2, 2009·The International Journal of Biochemistry & Cell Biology·Athina-Myrto ChioniMustafa B A Djamgoz
Jan 1, 2016·Journal of Neurochemistry·Sumita ChakrabartiAlan R Gintzler
Nov 1, 2008·Molecular Carcinogenesis·Shu-Chen HsiehSu Hao Lo
Aug 26, 2006·The International Journal of Biochemistry & Cell Biology·Banu IsbilenMustafa B A Djamgoz
Dec 3, 2014·The Journal of Surgical Research·Christian GeisAnna E Heverhagen
Oct 4, 2016·Expert Review of Clinical Immunology·Wang Sin Gina TanCostantino Pitzalis
Jan 1, 2009·Gene Regulation and Systems Biology·Mohammad Reza NouraniAbbas Ali Imani Fooladi
Dec 4, 2015·The Journal of Biological Chemistry·Wei YuJohn M Denu
Dec 7, 2006·Journal of Cellular Physiology·William J Brackenbury, Mustafa B A Djamgoz
Apr 19, 2020·Basic & Clinical Pharmacology & Toxicology·Nahit RizanerSeyhan Altun
Aug 18, 2020·Cellular and Molecular Neurobiology·Sumita ChakrabartiAlan R Gintzler
Mar 4, 2010·Journal of Cellular and Molecular Medicine·Lieke C J van den BerkRuurd Torensma
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Vincent IrawanToshiyuki Ikoma

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