Discrepant expression of microRNAs in transparent and cataractous human lenses

Investigative Ophthalmology & Visual Science
Changrui WuHui Zhang

Abstract

Age-related cataracts are considered to be a pathological condition that arise as senescence progresses. However, little is known about the function of microRNAs (miRNAs) in the formation of age-related cataracts. The purpose of this study was to identify possible differences in miRNA expression in the central epithelium of transparent and age-related cataractous human lenses. Microarrays were used to determine the miRNA expression profiles of both transparent and cataractous lenses. The results were analyzed by significance analyses performed by the microarray software, and the results were confirmed by stem-loop RT-PCR. Algorithms were used to predict the target genes of the differentially expressed miRNAs. Two hundred and six miRNAs were identified in all human lenses. The top eight miRNAs according to expression levels were miR-184, let-7b, miR-923, miR-1826, miR-125b, miR-1308, miR-26a, and miR-638 in transparent lenses. In contrast, the top eight miRNAs in cataractous lenses were miR-184, miR-1826, let-7b/c, miR-24, miR-23b, miR-923, and miR-23a. The expression levels of 20 miRNAs were increased and the levels of 12 miRNAs were decreased by more than 2-fold in transparent lenses relative to the levels in cataractous lense...Continue Reading

Citations

Apr 17, 2013·Cellular and Molecular Life Sciences : CMLS·Ivan ContePaola Bovolenta
Oct 15, 2014·Biochimica Et Biophysica Acta·Yu QinJinsong Zhang
Mar 20, 2016·Experimental Eye Research·Deepti Anand, Salil A Lachke
Jan 23, 2015·Bioinformatics·Thomas BleazardSam Griffiths-Jones
Mar 26, 2015·PloS One·Deepak P EdwardKhaled K Abu Amero
Nov 28, 2018·Journal of Cellular Physiology·Ying-Li Wei, Hao Sun
Jul 25, 2019·Journal of Cellular Biochemistry·Wenkai ZhouQichang Yan
Feb 6, 2020·Experimental and Therapeutic Medicine·Caimei XiuRuiying Song
Nov 2, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Feng ZhangBin Tong
Dec 21, 2018·Molecular Medicine Reports·Shu-Jun LiuYuan-Bin Li
Oct 19, 2017·International Journal of Molecular Medicine·Na ZhangYanhua Qi
Jan 8, 2021·Experimental Eye Research·Priscilla Sayami AkamineDânia Emi Hamassaki
May 1, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ewa Kosior-JareckaTomasz Żarnowski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Yan Li, Joram Piatigorsky
Molecular Therapy : the Journal of the American Society of Gene Therapy
Jikui ShenPeter A Campochiaro
Experimental Gerontology
Joel LancetaEugenia Wang
© 2021 Meta ULC. All rights reserved