Discrepin, a new peptide of the sub-family alpha-ktx15, isolated from the scorpion Tityus discrepans irreversibly blocks K+ -channels (IA currents) of cerebellum granular cells

Archives of Biochemistry and Biophysics
Gina D'SuzeGianfranco Prestipino

Abstract

A new peptide was purified from the venom of the Venezuelan scorpion Tityus discrepans, by high-performance liquid chromatography and its amino acid sequence was completed by Edman degradation and mass spectrometry analysis. It contains 38 amino acid residues with a molecular weight of 4177.7 atomic mass units, tightly folded by three disulfide bridges, and has a pyroglutamic acid at the N-terminal region. This peptide, named Discrepin, was shown to block preferentially the IA currents of the voltage-dependent K+ -channel of rat cerebellum granular cells in culture. The K+ -currents are inhibited in an apparently irreversible manner, whose 50% inhibitory effect is reached with a 190 nM toxin concentration. The systematic nomenclature proposed for this toxin is alpha-KTx15.6.

References

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Citations

Jun 17, 2014·Toxicon : Official Journal of the International Society on Toxinology·P DíazC Sevcik
May 23, 2014·Biochimica Et Biophysica Acta·Cristiana PiccoGianfranco Prestipino
Mar 17, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·G D'SuzeA Alagón
Feb 14, 2006·Pharmacology & Therapeutics·Susan I V Judge, Christopher T Bever
Jun 28, 2016·Toxicon : Official Journal of the International Society on Toxinology·Manuela Berto PuccaEliane Candiani Arantes

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