Apr 20, 2020

The effects of YKL-40 on angiogenic potential of HUVECs are partly mediated by syndecan-4

BioRxiv : the Preprint Server for Biology
Jianlei ZhengW. Sun


Objective: YKL-40, a secreted glycoprotein, has a role in promoting tumor angiogenesis through syndecan-1 receptor. As one of the members of syndecans family, syndecan-4 is also an important mediator for tube formation. However, the effects of YKL-40 on migration and tube formation of human umbilical vein cells (HUVECs) mediated by syndecan-4 receptor are unknown. Methods: HUVECs were transfected with lentivirus encoding syndecan-4 short hairpin (sh) RNA (lenti-synd4 shRNA) and the efficiency of transfection was measured using reverse transcription quantitative real time polymerase chain reaction (qRT-PCR) and flow cytometry. The effects of recombinant protein of YKL-40 on migration and angiogenesis of HUVECs adjusted by syndecan-4 were determined by wound healing and tube formation assay. The expression of protein kinase C (PKC) and extracellular signal regulated kinases (ERKs) 1 and 2 (ERK1/2) in HUVECs was measured using western blotting. Results: Transfection of lenti-synd4 shRNA significantly decreased the membrane protein expression of syndecan-4 in HUVECs. HUVECs transfected with lenti-synd4 shRNA remarkably inhibited the migration and tube formation of endothelial cells stimulated by recombinant protein of YKL-40. The l...Continue Reading

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