Discriminant quantitation of posttransplant hepatic reticuloendothelial function. The impact of ischemic preservation

Transplantation
A S KleinG B Bulkley

Abstract

This study focuses upon two discrete components of posttransplant hepatic reticuloendothelial system (RES) function-phagocytosis and killing of bacteria-under various conditions of ischemic preservation. We had previously reported that, following intravenous injection of rats with 51Cr and 125I double-labeled Escherichia coli, hepatic 51Cr levels can be used to reliably quantify hepatic phagocytic clearance of the bacteria from the blood (HPC), while the subsequent release of 125I from the liver accurately parallels hepatic bacterial killing. Here, Wistar rats were transplanted with syngeneic livers perfused with either normal saline (NS) or University of Wisconsin solution (UW) and stored at 4 degrees C for 1, 2, or 3 hr prior to implantation. Control rats underwent laparotomy and hepatic artery ligation. Using the double-labeled E coli assay, HPC was decreased in all transplanted animals when compared with controls, reaching a nadir on the third postoperative day (P < 0.05). In rats transplanted with livers preserved in NS, the fraction of phagocytosed organisms that were subsequently killed (hepatic killing efficiency=HKE) was increased to 142%, 129%, or 112% of normal following 1, 2, or 3 hr of cold ischemia, respectively; ...Continue Reading

References

Dec 1, 1991·Transplant International : Official Journal of the European Society for Organ Transplantation·K KanedaN Kamada
May 1, 1991·Reviews of Infectious Diseases·D L GeorgeC E Broelsch
Jan 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J C Caldwell-KenkelJ J Lemasters
Jan 1, 1989·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·G LahnborgJ Terblanche
Mar 1, 1988·Archives of Surgery·J O ColonnaR W Busuttil
Feb 1, 1989·Annals of Surgery·S J OntellT E Starzl
Jan 1, 1984·Annual Review of Immunology·D O Adams, T A Hamilton

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Citations

Oct 2, 2001·La Presse médicale·R Cursio
Jun 6, 2000·Journal of Thermal Biology·D A DuBoseI V Sils
Jun 1, 2006·World Journal of Surgery·Hasan AydedeSeda Vatansever
Mar 9, 2004·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Maria Rita Montenegro IsernSérgio Mies
Aug 28, 2003·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Nobuaki SakamotoAndrew S Klein
Feb 1, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·E C van HoornK van Norren
Jan 8, 1999·The Journal of Surgical Research·R CursioJ Mouiel
Jun 27, 2001·American Journal of Surgery·F Serracino-InglottR T Mathie

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