PMID: 8600028Feb 15, 1996Paper

Discrimination between different E-box-binding proteins at an endogenous target gene of c-myc

Genes & Development
L DesbaratsM Eilers

Abstract

c-myc plans a key role in regulating mammalian cell proliferation and apoptosis. The gene codes for a transcription factor, Myc, that belongs to the helix-loop-helix/leucine zipper (HLH/LZ) family of proteins. Myc heterodimerizes with a partner protein termed Max; the heterodimeric complex binds to CAC(G/A)TG (E-box) sequences and activates transcription from these sites. However, several other HLH/LZ proteins, including USF and TFE-3, bind to and trans-activate from the same element, yet have no documented effect on cell proliferation or apoptosis. Therefore, it is likely that mechanisms exist that discriminate between these proteins for activation of natural target genes of Myc. We now show that trans-activation from the E-box in the rat prothymosin-alpha intron enhancer is indeed specific for Myc, and identify both the distance from the start site of transcription and a second E-box element adjacent to that recognized by Myc as critical determinants of specificity. Surprisingly, transcription activation domains required for Myc to activate from this distal enhancer position differ from previously mapped domains and closely correlate with those domains essential for transformation. As observed in transformation assays, Myc an...Continue Reading

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Citations

Nov 13, 2002·Biochimica Et Biophysica Acta·Beate FrassTarik Möröy
Dec 11, 1999·Mutation Research·G FuhrmannG Krupitza
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S E HarperP A Sharp
Oct 6, 1997·The EMBO Journal·K PeukertM Eilers
Sep 2, 1998·The EMBO Journal·S Cohen-KaminskyP H Roméo
Feb 2, 2011·Molecular and Cellular Biology·Archana AnantharamanJörg Bungert
Jul 13, 2000·Molecular and Cellular Biology·L J WoodL M Resar
Oct 14, 2000·Annual Review of Cell and Developmental Biology·C GrandoriR N Eisenman
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·T C LeeE B Ziff
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·K E BoydP J Farnham
Nov 22, 2005·Biochimica Et Biophysica Acta·Janka SepelakovaJuraj Kopacek
Apr 27, 2004·Journal of Cellular Biochemistry·A StojanovaR J Germinario
Aug 9, 2018·The Journal of Biological Chemistry·Huabo WangEdward V Prochownik
Aug 8, 1996·Nature·K GalaktionovD Beach
Jun 23, 1999·Oncogene·G F Claassen, S R Hann

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