Discrimination Between Infant and Adult Bloodstains Using Micro-Raman Spectroscopy: A Preliminary Study

Journal of Forensic Sciences
J FujiharaH Takeshita

Abstract

In the present study, we used micro-Raman spectroscopy with high-resolution analysis to discriminate between bloodstains from infants and bloodstains from adults. Raman peaks were detected at 674, 754, 976, 1002, 1105, 1127, 1176, 1248, 1340, 1368, 1390, 1560, and 1611 cm-1 ; these peaks were derived from hemoglobin, albumin, and glucose. However, a peak was obtained at 1105 cm-1 , which was assigned to histidine; this peak was observed only for bloodstains from adults. Human adult hemoglobin (HbA) is composed of an α2 β2 tetramer structure, whereas human fetal hemoglobin (HbF) is composed of an α2 γ2 . Therefore, the lack of a Raman peak at 1105 cm-1 in bloodstains from infants indicates the possibility of two histidine substitutions (His116Ile and His143Ser) in the γ chain of HbF. This study discriminates between bloodstains from infants and bloodstains from adults using micro-Raman spectroscopy, with beneficial implications in forensic science.

References

Mar 1, 1973·Journal of Medical Genetics·P A Lorkin
Jun 1, 1970·The Journal of Clinical Investigation·H F Bunn, R W Briehl
Sep 27, 2008·Forensic Science International : Synergy·Kelly Virkler, Igor K Lednev
Oct 17, 2009·Analytical and Bioanalytical Chemistry·Kelly Virkler, Igor K Lednev
May 11, 2010·PloS One·Aseefhali BankapurDeepak Mathur
Jan 7, 2011·Forensic Science International : Synergy·Samantha BoydSarah J Seashols
Jan 19, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Guangyong ZhuXueliang Wan
May 9, 2014·Forensic Science International : Synergy·Gregory McLaughlin, Igor K Lednev
Oct 29, 2014·Analytical Chemistry·Gregory McLaughlinIgor K Lednev
Jun 6, 2016·International Journal of Legal Medicine·J FujiharaH Takeshita
Nov 15, 2016·Forensic Science International : Synergy·Ilan FeineIddo Pinkas

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