Discrimination between Mycoplasma and Acholeplasma species of bovine origin using digitonin disc diffusion assay, nisin disc diffusion assay, and conventional polymerase chain reaction

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
S BoonyayatraT E Besser

Abstract

Microbiological culture of milk samples has been used as a standard diagnosis for Mycoplasma mastitis. This technique is effective in isolating mollicutes that are Mycoplasma-like; however, isolates may be misinterpreted as Acholeplasma species, which are indistinguishable from Mycoplasma species by culture. A study to contrast the abilities of 2 culture-based tests, digitonin and nisin disc diffusion assays and a conventional polymerase chain reaction (PCR) technique, to discriminate between Mycoplasma and Acholeplasma was performed using 16S ribosomal RNA gene partial sequencing as the gold standard of comparison. A total of 288 bovine mollicute field isolates (248 from milk and 40 from other organ sites) and 13 reference strains were tested. Results obtained from the digitonin disc diffusion assay when it was performed with all field isolates were 92.7% and 99.0% in agreement with the gold standard using 5 mm and 3 mm of zone of growth inhibition as thresholds, respectively. Considering only milk isolates, agreements between the digitonin disc diffusion assay with the gold standard were 97.2% and 100% using 5 mm and 3 mm of zone of growth inhibition as thresholds, respectively. Culture identification using the nisin disc dif...Continue Reading

References

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Aug 27, 2005·Journal of the American Veterinary Medical Association·Mary K BiddleClive C Gay

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Citations

Jun 6, 2012·The Veterinary Clinics of North America. Food Animal Practice·Lawrence K Fox
Dec 3, 2015·The Veterinary Journal·Inna LysnyanskySharon Levisohn
Sep 1, 2015·Evolution; International Journal of Organic Evolution·James R MorrisAndrew Berry
Nov 28, 2017·BMC Veterinary Research·Abd Al-Bar Al-FarhaKiro Petrovski
Jun 28, 2018·Tropical Animal Health and Production·Aqeela Ashraf, Muhammad Imran
Apr 20, 2018·Journal of Veterinary Internal Medicine·Alysia M ParkerJohn K House

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Methods Mentioned

BETA
PCR

Software Mentioned

Basic Local Alignment Search Tool ( BLAST
BLAST

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