Apr 6, 2016

Discrimination between Onchocerca volvulus and O. ochengi filarial larvae in Simulium damnosum s.l. and their distribution throughout central Ghana using a versatile high-resolution speciation assay

BioRxiv : the Preprint Server for Biology
Stephen R DoyleWarwick N Grant

Abstract

Background Genetic surveillance of the human filarial parasite, Onchocerca volvulus, from onchocerciasis endemic regions will ideally focus on genotyping individual infective larval stages collected from their intermediate host, Simuliid blackflies. However, blackflies also transmit other Onchocerca species, including the cattle parasite O. ochengi, which are difficult to distinguish from the human parasite based on morphological characteristics alone. This study describes a versatile approach to discriminate between O. volvulus and O. ochengi that is demonstrated using parasite infective larvae dissected from blackflies. Results A speciation assay was designed based on genetic differentiation between O. volvulus and O. ochengi mitochondrial genome sequences that can be performed in high-throughput high-resolution melt- or lower throughput conventional RFLP analyses. This assay was validated on 185 Onchocerca larvae dissected from blackflies captured from 14 communities in Ghana throughout 2011-13. The frequency of O. ochengi was approximately 67% of all larvae analyzed, which is significantly higher than previously reported in this region. Furthermore, the species distribution was not uniform throughout the study region, with ...Continue Reading

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Mentioned in this Paper

Genetic Drift
Study
Filarial worm
Simulium damnosum
Onchocerciasis
VEPH1 gene
Onchocerca ochengi
Larva
West (Direction)
Onchocerca

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