Apr 6, 2016

Discrimination between Onchocerca volvulus and O. ochengi filarial larvae in Simulium damnosum s.l. and their distribution throughout central Ghana using a versatile high-resolution speciation assay

BioRxiv : the Preprint Server for Biology
Stephen R DoyleWarwick N Grant


Background Genetic surveillance of the human filarial parasite, Onchocerca volvulus, from onchocerciasis endemic regions will ideally focus on genotyping individual infective larval stages collected from their intermediate host, Simuliid blackflies. However, blackflies also transmit other Onchocerca species, including the cattle parasite O. ochengi, which are difficult to distinguish from the human parasite based on morphological characteristics alone. This study describes a versatile approach to discriminate between O. volvulus and O. ochengi that is demonstrated using parasite infective larvae dissected from blackflies. Results A speciation assay was designed based on genetic differentiation between O. volvulus and O. ochengi mitochondrial genome sequences that can be performed in high-throughput high-resolution melt- or lower throughput conventional RFLP analyses. This assay was validated on 185 Onchocerca larvae dissected from blackflies captured from 14 communities in Ghana throughout 2011-13. The frequency of O. ochengi was approximately 67% of all larvae analyzed, which is significantly higher than previously reported in this region. Furthermore, the species distribution was not uniform throughout the study region, with ...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Genetic Drift
Filarial worm
Simulium damnosum
VEPH1 gene
Onchocerca ochengi
West (Direction)

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.