Discrimination between two possible reaction sequences that create potential risk of generation of deleterious radicals by cytochrome bc₁. Implications for the mechanism of superoxide production.

Biochimica Et Biophysica Acta
Marcin SarewiczArtur Osyczka

Abstract

In addition to its bioenergetic function of building up proton motive force, cytochrome bc₁ can be a source of superoxide. One-electron reduction of oxygen is believed to occur from semiquinone (SQ(o)) formed at the quinone oxidation/reduction Q(o) site (Q(o)) as a result of single-electron oxidation of quinol by the iron-sulfur cluster (FeS) (semiforward mechanism) or single-electron reduction of quinone by heme b(L) (semireverse mechanism). It is hotly debated which mechanism plays a major role in the overall production of superoxide as experimental data supporting either reaction exist. To evaluate a contribution of each of the mechanisms we first measured superoxide production under a broad range of conditions using the mutants of cytochrome bc₁ that severely impeded the oxidation of FeS by cytochrome c₁, changed density of FeS around Q(o) by interfering with its movement, or combined these two effects together. We then compared the amount of generated superoxide with mathematical models describing either semiforward or semireverse mechanism framed within a scheme assuming competition between the internal reactions at Q(o) and the leakage of electrons on oxygen. We found that only the model of semireverse mechanism correctl...Continue Reading

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Citations

Jun 29, 2011·The Journal of Biological Chemistry·Casey L QuinlanMartin D Brand
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·V G Grivennikova, A D Vinogradov
Feb 25, 2014·Biochimica Et Biophysica Acta·Stefan DröseIlka Wittig
Oct 13, 2015·Free Radical Biology & Medicine·Darío E IglesiasAlberto Boveris
Jun 10, 2014·Biochimica Et Biophysica Acta·F GuillaudE Klipp
Oct 9, 2014·Biophysical Journal·S Saif HasanWilliam A Cramer
Dec 28, 2012·Biochimica Et Biophysica Acta·Lea Bleier, Stefan Dröse
Jun 22, 2016·Biochimica Et Biophysica Acta·Lucie BergdollFrauke Baymann
Jul 23, 2016·Journal of the American Chemical Society·Peter Husen, Ilia A Solov'yov
Jan 25, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marcin SarewiczArtur Osyczka
Jan 31, 2018·Photosynthesis Research·Ryan M TaylorTerry M Bricker
Dec 7, 2018·The Journal of Biological Chemistry·Urszula GodlewskaJoanna Cichy
Sep 19, 2019·Oxidative Medicine and Cellular Longevity·Jin-Xin TaoXin-Gen Zhu
Dec 23, 2020·Free Radical Biology & Medicine·Jakub PagaczArkadiusz Borek
Jun 8, 2021·Frontiers in Chemistry·Peter Husen, Ilia A Solov'yov

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