Discrimination of DNA and RNA distribution in a mammalian cell by scanning transmission soft X-ray microscopy

Journal of X-ray Science and Technology
Kunio ShinoharaShigenobu Toné

Abstract

Soft X-ray spectromicroscopy was applied to study the distribution of DNA and RNA in a mammalian cell at the spatial resolution of 400 nm. The relative distribution of DNA and RNA was examined by the SVD (singular value decomposition) method in aXis2000 program using combined full spectra of DNA and RNA at the absorption edge regions of carbon, nitrogen and oxygen. The absorption of nucleic acid was evaluated using 1s-π* transitions in the NEXAFS spectra at the nitrogen K absorption edge and distributed to DNA and RNA according to the relative level obtained above. The present results revealed the usefulness of the SVD method to discriminate closely related molecules such as DNA and RNA.

References

May 1, 1996·Journal of Structural Biology·X ZhangJ Kirz
Jan 3, 2007·Biochemistry·Shotaro InoueTakashi Ohyama
Jan 11, 2007·The FEBS Journal·Noriyuki SumidaTakashi Ohyama
Dec 21, 2012·Nucleic Acids Research·Jun-ichi Nishikawa, Takashi Ohyama
Nov 7, 2013·Nanoscale Research Letters·Zhong YangquanweiChithra Karunakaran
Jul 23, 2014·FEMS Microbiology Letters·Jan JamroskovicRizlan Bernier-Latmani

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Methods Mentioned

BETA
X-ray

Software Mentioned

aXis2000
spect2D
RNAspect
spect2R

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