Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.

Proceedings of the National Academy of Sciences of the United States of America
H AkagiR Miledi

Abstract

Three synthetic oligodeoxynucleotides complementary to different parts of an RNA encoding a glycine receptor subunit were used to discriminate heterogenous mRNAs coding for glycine receptors in adult and neonatal rat spinal cord. Injection of the three antisense oligonucleotides into Xenopus oocytes specifically inhibited the expression of glycine receptors by adult spinal cord mRNA. In contrast, the antisense oligonucleotides were much less potent in inhibiting the expression of glycine receptors encoded by neonatal spinal cord mRNA. Northern blot analysis revealed that the oligonucleotides hybridized mostly to an adult cord transcript of approximately 10 kilobases in size. This band was also present in neonatal spinal cord mRNA but its density was about one-fourth of the adult cord message. There was no intense band in the low molecular weight position (approximately 2 kilobases), the existence of which was expected from electrophysiological studies with size-fractionated mRNA of neonatal spinal cord. Our results suggest that in the rat spinal cord there are at least three different types of mRNAs encoding functional strychnine-sensitive glycine receptors.

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Citations

Aug 15, 1993·Journal of Neuroscience Research·C MatuteR Miledi
Jul 1, 1993·Brain Research. Molecular Brain Research·G PollioA Maggi
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D S Ragsdale, R Miledi
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·A MoralesR Miledi
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Mar 15, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Guadalupe García-AlcocerAtaúlfo Martínez-Torres
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Oct 19, 2000·Experimental Cell Research·C D CorporeauM Penrad-Mobayed

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