Discrimination of large maltooligosaccharides from isobaric dextran and pullulan using ion mobility mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Abdul M RashidStephen Bornemann

Abstract

Ion mobility mass spectrometry (IMMS) has previously been shown to resolve small isobaric oligosaccharides, but larger alpha-oligoglucans are also abundant in biology and are of industrial importance. If conformational differences between such isomers are retained in the gas phase, IMMS could be used to address questions in biology and industry. Negative mode electrospray ionization (ESI) travelling-wave IMMS was used to resolve large isobaric α-glucan ions on the basis of their different gas-phase conformations. α,ω-Dicarboxy-terminated polystyrene was used to calibrate the instrument allowing the collision cross-sections (CCSs) of ions to be determined. α-1,4-Linked maltooligosaccharides with a degree of polymerisation of up to 35 could be discriminated from α-1,6-linked dextran and α-1,4/1,6-linked pullulan using IMMS. Fragmentation spectra of ions separated by IMMS could also distinguish isomers. Two conformational isomers of maltohexaose were resolvable by IMMS, likely reflecting extended and V6 helical conformations. IMMS was also able to identify a product within a mixture of maltooligosaccharides treated with the potential anti-tuberculosis drug target Mycobacterium tuberculosis GlgB branching enzyme. Biological samples...Continue Reading

References

Aug 6, 1998·Journal of the American Society for Mass Spectrometry·A E CountermanD E Clemmer
Jul 25, 2000·Critical Reviews in Biochemistry and Molecular Biology·J Kossmann, J Lloyd
Nov 8, 2005·Physical Chemistry Chemical Physics : PCCP·Lan JinDusan Uhrín
Sep 29, 2006·Protein Expression and Purification·Saurabh K GargPushpa Agrawal
Feb 2, 2008·Journal of Proteome Research·D IsailovicD E Clemmer
Sep 2, 2008·Journal of the American Society for Mass Spectrometry·Manolo D PlasenciaDavid E Clemmer
Nov 11, 2008·Chemical Communications : Chem Comm·Larissa S Fenn, John A McLean
Jun 30, 2009·Analytical and Bioanalytical Chemistry·Maolei ZhuHerbert H Hill
Nov 11, 2009·Rapid Communications in Mass Spectrometry : RCM·Shusaku DaikokuOsamu Kanie
Mar 23, 2010·Nature Chemical Biology·Rainer KalscheuerWilliam R Jacobs
Nov 30, 2010·Physical Chemistry Chemical Physics : PCCP·Larissa S Fenn, John A McLean
Jul 1, 1997·Analytical Chemistry·Y Liu, D E Clemmer
Jul 30, 2011·Applied Microbiology and Biotechnology·Kuan-Chen ChengJeffrey M Catchmark
Sep 15, 2011·The Journal of Biological Chemistry·Karl SysonStephen Bornemann
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Steven M ZuckerDavid E Clemmer
Jan 22, 2013·Analytical Chemistry·Hongli LiHerbert H Hill

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Citations

Jul 9, 2014·Journal of the American Society for Mass Spectrometry·Kihyun KimHugh I Kim
Jul 25, 2015·Journal of the American Society for Mass Spectrometry·David J HarveyJim H Scrivens
May 26, 2016·Biochemistry·Abdul M RashidStephen Bornemann
Apr 25, 2017·Angewandte Chemie·Johanna Hofmann, Kevin Pagel
Oct 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuqing MuVito Ferro
Feb 9, 2021·Frontiers in Plant Science·Arely V Pérez-LópezJosé J Ordaz-Ortiz
Mar 25, 2021·Rapid Communications in Mass Spectrometry : RCM·Razieh Parchami, Mahmoud Tabrizchi
Dec 31, 2016·Analytical Chemistry·Jody C MayJohn A McLean

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