Discrimination of methylated and nonmethylated region of a colorectal cancer related promoter using fluorescence enhancement of gold nanocluster at intrastrand of a 9C-loop

Methods and Applications in Fluorescence
Hossein SoltaninejadMohammad Reza Ganjali

Abstract

Among epigenetic modifications of DNA, methylation of cytosine at its carbon 5 is the most common mark that is usually associated with gene silencing in human. Determining whether a particular DNA molecule is methylated or not, is an indispensable task in many epigenetic investigations. Presenting detection methods with less labor-intensive and time-consuming procedures has substantial value. Here a facile method based on gold nanocluster (AuNCs) fluorescence enhancement is presented. Target sequences were selected from Sept9 promoter region as its hypermethylation is demonstrated as a reliable biomarker of colorectal cancer. DNA probe was complementary to a 25 nucleotide of the target region and possessed 9 additional cytosines in the middle to allow the formation of AuNCs. Probe-AuNCs strands were first hybridized with methylated and non-methylated targets separately, and then their fluorescence intensities were recorded. Fluorescence intensity was higher with methylated targets than non-methylated one. Applying silver ions reversed the situation and fluorescence intensities of non-methylated DNA got higher than methylated one.

References

Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Behe, G Felsenfeld
Aug 11, 1994·Nucleic Acids Research·S J ClarkM Frommer
Jul 4, 2002·Methods : a Companion to Methods in Enzymology·Peter M WarneckeSusan J Clark
Feb 6, 2007·Bioorganic & Medicinal Chemistry Letters·Kazuo Tanaka, Akimitsu Okamoto
Apr 25, 2007·Nucleic Acids Research·Warren A Kibbe
Feb 5, 2009·Nucleic Acids Research·Jaroslav KyprMichaela Vorlícková
Jul 7, 2010·Nature Reviews. Cardiology·José M Ordovás, Caren E Smith
May 18, 2011·Genes & Development·Aimée M Deaton, Adrian Bird
Mar 7, 2012·Cancer Letters·Karen ChiamTina Bianco-Miotto
Mar 17, 2012·The Analyst·Chenchen GeLingwen Zeng
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 17, 2012·Nucleic Acids Research·Laurence R MeyerW James Kent
Aug 19, 2014·Nature Neuroscience·Philip L De JagerDavid A Bennett
Oct 3, 2014·Analytical Chemistry·Li-Yi ChenHuan-Tsung Chang
Nov 13, 2014·Clinical Chemistry·Ellen HeitzerJochen B Geigl
Jan 9, 2015·The Journal of Allergy and Clinical Immunology·Hani Harb, Harald Renz
Mar 10, 2017·Methods and Applications in Fluorescence·Morteza HosseiniMohammad Reza Ganjali
Jun 13, 2017·Chemical Communications : Chem Comm·Fei MaChun-Yang Zhang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.