Discrimination of repetitive sequences polymorphism in Secale cereale by genomic in situ hybridization-banding

Journal of Integrative Plant Biology
Jian-Ping ZhouZhenglong Ren

Abstract

Genomic in situ hybridization banding (GISH-banding), a technique slightly modified from conventional GISH, was used to probe the Chinese native rye (Secale cereale L.) DNA, and enabled us to visualize the individual rye chromosomes and create a universal reference karyotype of the S. cereale chromosome 1R to 7R. The GISH-banding approach used in the present study was able to discriminate S. cereale chromosomes or segments in the wheat (Triticum aestivum L.) background, including the Triticale, wheat-rye addition and translocation lines. Moreover, the GISH-banding pattern of S. cereale subsp. Afghanicum chromosomes was consistent with that of Chinese native rye cv. Jingzhou rye; whereas the GISH-banding pattern of Secale vavilovii was different from that of S. cereale, indicating that GISH-banding can be used to study evolutionary polymorphism in species or subspecies of Secale. In addition, the production and application of GISH-banding to the study of adenine-thymine-riched heterochromatin is discussed.

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Citations

Jun 14, 2013·Journal of Integrative Plant Biology·Qiong WuKunbo Wang
Dec 9, 2014·Genome Génome / Conseil National De Recherches Canada·Wenping GongZujun Yang
Jul 17, 2014·Journal of Applied Genetics·Diána IcsóGabriella Linc
May 5, 2020·Genome Génome / Conseil National De Recherches Canada·Egizia Falistocco, Nicoletta Ferradini

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