PMID: 6982880Sep 1, 1982Paper

Discrimination of slow growth from non-survival among small colonies of diploid Syrian hamster cells after chromosome damage induced by a range of x-ray doses

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
G P JoshiC A Shaw

Abstract

Synchronous samples of cultured diploid Syrian hamster cells (BHK 21 C13/A3) were obtained by mitotic selection, transferred to an observation chamber and given a single X-ray dose in the range 0.2 to 3.8 Gy. Each cell's radiation response was followed by visual observations of its progress alive through post-radiation mitosis (M1), and subsequently of its clonogenicity, by methods already published (Grote et al. 1981 a, b; Joshi et al. 1982a). Our recent paper about the same cell samples (Joshi et al. 1982b) showed that the probability of reaching M1 is nearly unity in controls and over the whole dose range (mean greater than 0.99). The present paper describes the clonogenicity of each sample, based on five daily cell counts at the site of each initial cell. The frequency of viable colonies falls from 98 per cent for unirradiated cells to 8 per cent in the 3.8 Gy sample, but the proportion of these which grow slowly rises from 3 to more than 70 per cent. There was substantial overlap in cell numbers reached by larger abortive colonies and smaller slow-growth colonies, and many of the later had not reached 50 cells at the last count at 5 1/2 days. Impaired colony growth (slow growth or stop growth) was strongly correlated with ...Continue Reading

References

May 1, 1970·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·K R Trott, O Hug
May 1, 1956·The Journal of Experimental Medicine·T T PUCK, P I MARCUS
Sep 26, 1964·Nature·M STOKER, I MACPHERSON

❮ Previous
Next ❯

Citations

Jan 1, 1986·Radiation and Environmental Biophysics·K H von WangenheimL E Feinendegen
Jul 1, 1983·The British Journal of Radiology·J F Fowler
Oct 1, 1994·International Journal of Radiation Biology·I Szumiel
Jan 26, 2012·Mutation Research·Carmel Mothersill, Colin Seymour
May 1, 1985·Cell and Tissue Kinetics·W A Brock, M Williams
Jan 18, 2006·Mutation Research·Carmel Mothersill, C B Seymour
Jan 1, 1984·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A Michalowski
Oct 1, 1993·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·M TubianaE Frindel
Jun 24, 2003·Physics in Medicine and Biology·Marie-Claude BistonJacques Balosso
Mar 2, 2019·International Journal of Radiation Biology·Carmel Mothersill, Colin Seymour
Nov 15, 2017·Radiation Research·Gabriel AdrianLars Ekblad
Jul 1, 1987·Reviews on Environmental Health·B A Kihlman, H C Andersson
Dec 3, 2020·Physics in Medicine and Biology·Robin A KochEmanuel Bahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.