Discrimination of Stem Cell Status after Subjecting Cynomolgus Monkey Pluripotent Stem Cells to Naïve Conversion

Scientific Reports
Arata HondaMitinori Saitou


Experimental animal models have played an indispensable role in the development of human induced pluripotent stem cell (iPSC) research. The derivation of high-quality (so-called "true naïve state") iPSCs of non-human primates enhances their application and safety for human regenerative medicine. Although several attempts have been made to convert human and non-human primate PSCs into a truly naïve state, it is unclear which evaluation methods can discriminate them as being truly naïve. Here we attempted to derive naïve cynomolgus monkey (Cm) (Macaca fascicularis) embryonic stem cells (ESCs) and iPSCs. Several characteristics of naïve Cm ESCs including colony morphology, appearance of naïve-related mRNAs and proteins, leukaemia inhibitory factor dependency, and mitochondrial respiration were confirmed. Next, we generated Cm iPSCs and converted them to a naïve state. Transcriptomic comparison of PSCs with early Cm embryos elucidated the partial achievement (termed naïve-like) of their conversion. When these were subjected to in vitro neural differentiation, enhanced differentiating capacities were observed after naïve-like conversion, but some lines exhibited heterogeneity. The difficulty of achieving contribution to chimeric mou...Continue Reading


Apr 11, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Arata Honda
Nov 15, 2019·Biology of Reproduction·Yoshitake SakaiMitinori Saitou
May 21, 2020·Cellular Reprogramming·Monika Nowak-ImialekHeiner Niemann
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Related Concepts

Regenerative Medicine
Pluripotent Stem Cells
Mitochondrial Inheritance
Colony Morphology
Phage Display Peptide Library
Enhanced S-Cone Syndrome
Cell Respiration
Chimera Organism

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