PMID: 8595703Dec 1, 1995Paper

Discrimination of Streptococcus pneumoniae from other upper respiratory tract streptococci by arbitrarily primed PCR

Clinical Biochemistry
T O MessmerR R Facklam

Abstract

In the clinical laboratory, identification of Streptococcus pneumoniae can be confused with other streptococci. Conventional biochemical tests such as optochin sensitivity and bile solubility can give inconsistent results. This report presents a method to distinguish true S. pneumoniae from other upper respiratory tract streptococci when conventional tests fail. We used arbitrarily primed polymerase chain reaction with the single primer M13 universal as a method to distinguish S. pneumoniae from other upper respiratory tract streptococci. The fingerprint pattern of S. pneumoniae was established by amplifying DNA of S. pneumoniae type strains 1-48 and of other common upper respiratory tract streptococci at three different DNA concentrations with the single primer M13 universal. From these type strains, a common arbitrarily primed-polymerase chain reaction pattern was identified characterized by two predominant bands of equal intensity at 800 base pairs and at 1100 base pairs. Fingerprint patterns of viridans streptococci were easily distinguishable from those of S. pneumoniae. Many of the clinical isolates used in this study were equivocal by conventional tests but were distinguishable by their fingerprint patterns. Our results ...Continue Reading

References

Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland

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Citations

Apr 16, 1999·Oral Microbiology and Immunology·J D Rudney, C J Larson
Aug 18, 2004·Diagnostic Microbiology and Infectious Disease·Trudy O MessmerRichard R Facklam
Feb 24, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Renata Chaves AlbuquerqueMarina Baquerizo Martinez

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