Discriminatory recognition of membrane phospholipids by lysine-49-phospholipases A2 from Trimeresurus flavoviridis venom

Journal of Molecular Recognition : JMR
Y ShimohigashiM Ohno

Abstract

Basic proteins I and II (BP-I and BP-II) isolated from Trimeresurus flavoviridis venom, which are classified into a group of lysine-49-phospholipases A2 (Lys-49-PLA2), exhibited only limited lipolytic activity for the mixed micelles of various phospholipids. Based on the finding that BP-II elicits a strong contraction of guinea pig ileum due to the release of arachidonic acid, BP-II together with BP-I has been tested for their interaction with artificial phospholipid bilayer membranes. The dye leakage experiments indicated that BP-II interacts strongly with liposomes of beta-arachidonoyl-gamma-stearoyl-L-alpha-phosphatidylcholine. The perturbation of liposomes was observed only in the Ca(2+)-containing buffer, and as demonstrated by HPLC analyses, accompanied by the release of arachidonic acid. The concentration of Ca2+ which gave a half maximal activity of BP-II was 3.0 x 10(-4) M, suggesting that the affinity of BP-II for Ca2+ is more than 10 times stronger than that of BP-II without liposomes. These observations clearly show that Lys-49-PLA2 of BP-II is the enzyme responsible for the hydrolysis of membrane phospholipids and that Ca2+ is essential for such enzymatic activity. The interaction of BP-I with liposomes was much we...Continue Reading

References

Sep 5, 1978·Journal of Molecular Biology·B W DijkstraP J Vandermaelen
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T OgawaM Ohno
Feb 1, 1991·Archives of Biochemistry and Biophysics·B FrancisI I Kaiser
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·H C Huang
Jan 1, 1980·Toxicon : Official Journal of the International Society on Toxinology·S Sadahiro, T Omori-Satoh
Feb 12, 1981·Nature·B W DijkstraK H Kalk
Jun 1, 1940·The Biochemical Journal·R J Allen

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