Disease-driving CD4+ T cell clonotypes persist for decades in celiac disease

The Journal of Clinical Investigation
Louise F RisnesLudvig M Sollid

Abstract

Little is known about the repertoire dynamics and persistence of pathogenic T cells in HLA-associated disorders. In celiac disease, a disorder with a strong association with certain HLA-DQ allotypes, presumed pathogenic T cells can be visualized and isolated with HLA-DQ:gluten tetramers, thereby enabling further characterization. Single and bulk populations of HLA-DQ:gluten tetramer-sorted CD4+ T cells were analyzed by high-throughput DNA sequencing of rearranged TCR-α and -β genes. Blood and gut biopsy samples from 21 celiac disease patients, taken at various stages of disease and in intervals of weeks to decades apart, were examined. Persistence of the same clonotypes was seen in both compartments over decades, with up to 53% overlap between samples obtained 16 to 28 years apart. Further, we observed that the recall response following oral gluten challenge was dominated by preexisting CD4+ T cell clonotypes. Public features were frequent among gluten-specific T cells, as 10% of TCR-α, TCR-β, or paired TCR-αβ amino acid sequences of total 1813 TCRs generated from 17 patients were observed in 2 or more patients. In established celiac disease, the T cell clonotypes that recognize gluten are persistent for decades, making up fixe...Continue Reading

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Nov 21, 2018·The Journal of Biological Chemistry·Shiva Dahal-KoiralaLudvig M Sollid
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Datasets Mentioned

BETA
SRP102399
SRP102402

Methods Mentioned

BETA
biopsy
biopsies
density gradient centrifugation
FCS
Flow Cytometry
PCR

Software Mentioned

HighV
pRESTO
FlowJo
QUEST

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