Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants

The Plant Cell
G WuD M Shah

Abstract

Plant defense responses to pathogen infection involve the production of active oxygen species, including hydrogen peroxide (H2O2). We obtained transgenic potato plants expressing a fungal gene encoding glucose oxidase, which generates H2O2 when glucose is oxidized. H2O2 levels were elevated in both leaf and tuber tissues of these plants. Transgenic potato tubers exhibited strong resistance to a bacterial soft rot disease caused by Erwinia carotovora subsp carotovora, and disease resistance was sustained under both aerobic and anaerobic conditions of bacterial infection. This resistance to soft rot was apparently mediated by elevated levels of H2O2, because the resistance could be counteracted by exogenously added H2O2-degrading catalase. The transgenic plants with increased levels of H2O2 also exhibited enhanced resistance to potato late blight caused by Phytophthora infestans. The development of lesions resulting from infection by P. infestans was significantly delayed in leaves of these plants. Thus, the expression of an active oxygen species-generating enzyme in transgenic plants represents a novel approach for engineering broad-spectrum disease resistance in plants.

References

May 1, 1992·Plant Molecular Biology·N T Keen
Jun 1, 1991·Journal of Bioenergetics and Biomembranes·A Vianello, F Macrì
Jan 1, 1990·Annual Review of Biochemistry·D J Bowles
Oct 26, 1987·Nucleic Acids Research·R D RichinsR J Shepherd
Dec 1, 1994·Plant Molecular Biology·D F Klessig, J Malamy

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Citations

Aug 31, 1999·Archives of Insect Biochemistry and Physiology·H EichenseerG W Felton
Jun 1, 2006·Mycotoxin Research·K RajasekaranT E Cleveland
Mar 12, 2008·Applied Microbiology and Biotechnology·Chun Ming WongXiao Dong Chen
Mar 24, 2004·Trends in Biotechnology·Yaroslav I KorpanAnna V El'skaya
Jan 1, 1997·Free Radical Biology & Medicine·C R McClung
Feb 7, 2001·Current Opinion in Biotechnology·C M Rommens, G M Kishore
Apr 1, 1997·Current Opinion in Biotechnology·D M Shah
Mar 14, 2000·Current Opinion in Plant Biology·L S Melchers, M H Stuiver
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Denny G MellershMichele C Heath
Jan 24, 2004·Journal of Experimental Botany·Christophe BaillyFrançoise Corbineau
Feb 22, 2005·Molecular Plant-microbe Interactions : MPMI·Wolfgang MoederDaniel F Klessig
Jun 9, 2007·Molecular Plant-microbe Interactions : MPMI·Zenaida V MagbanuaDawn S Luthe
Sep 1, 1996·Plant Physiology·J A FrugoliC R McClung
Oct 1, 1996·The Plant Cell·K E Hammond-Kosack, J D Jones
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·N N GesslerT A Belozerskaya
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Jan 1, 1997·Annual Review of Genetics·J GlazebrookF M Ausubel
Jan 20, 2004·Bioscience, Biotechnology, and Biochemistry·Yoshimitsu Takakura, Shigeru Kuwata
Oct 13, 2000·Biological Chemistry·H Sandermann

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