Disentangling pectic homogalacturonan and rhamnogalacturonan-I polysaccharides: Evidence for sub-populations in fruit parenchyma systems

Food Chemistry
Valérie CornuaultJ Paul Knox

Abstract

The matrix polysaccharides of plant cell walls are diverse and variable sets of polymers influencing cell wall, tissue and organ properties. Focusing on the relatively simple parenchyma tissues of four fruits - tomato, aubergine, strawberry and apple - we have dissected cell wall matrix polysaccharide contents using sequential solubilisation and antibody-based approaches with a focus on pectic homogalacturonan (HG) and rhamnogalacturonan-I (RG-I). Epitope detection in association with anion-exchange chromatography analysis indicates that in all cases solubilized polymers include spectra of HG molecules with unesterified regions that are separable from methylesterified HG domains. In highly soluble fractions, RG-I domains exist in both HG-associated and non-HG-associated forms. Soluble xyloglucan and pectin-associated xyloglucan components were detected in all fruits. Aubergine glycans contain abundant heteroxylan epitopes, some of which are associated with both pectin and xyloglucan. These profiles of polysaccharide heterogeneity provide a basis for future studies of more complex cell and tissue systems.

Citations

Mar 23, 2018·International Journal of Molecular Sciences·Mateusz Majda, Stéphanie Robert
Jan 24, 2019·International Journal of Molecular Sciences·Matteo ChialvaRaffaella Balestrini
May 29, 2020·The Plant Journal : for Cell and Molecular Biology·Andrew F GallowayPaul Knox
Oct 15, 2019·Critical Reviews in Food Science and Nutrition·Dongmei WuShiguo Chen
Apr 25, 2020·Planta·María Moneo-SánchezIgnacio Martín
Jul 12, 2019·Frontiers in Plant Science·Romina PedreschiReinaldo Campos-Vargas
Aug 25, 2018·Current Opinion in Plant Biology·Daniel J Cosgrove
Apr 1, 2021·The Plant Journal : for Cell and Molecular Biology·Sukhita SathitnaithamSupachai Vuttipongchaikij

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Methods Mentioned

BETA
transgenic
ELISA
size exclusion chromatography
ELISAs
dissection

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