Disentangling thermal stress responses in a reef-calcifier and its photosymbionts by shotgun proteomics

Scientific Reports
Marleen StuhrHildegard Westphal

Abstract

The proliferation of key marine ecological engineers and carbonate producers often relies on their association with photosymbiotic algae. Evaluating stress responses of these organisms is important to predict their fate under future climate projections. Physiological approaches are limited in their ability to resolve the involved molecular mechanisms and attribute stress effects to the host or symbiont, while probing and partitioning of proteins cannot be applied in organisms where the host and symbiont are small and cannot be physically separated. Here we apply a label-free quantitative proteomics approach to detect changes of proteome composition in the diatom-bearing benthic foraminifera Amphistegina gibbosa experimentally exposed to three thermal-stress scenarios. We developed a workflow for protein extraction from less than ten specimens and simultaneously analysed host and symbiont proteomes. Despite little genomic data for the host, 1,618 proteins could be partially assembled and assigned. The proteomes revealed identical pattern of stress response among stress scenarios as that indicated by physiological measurements, but allowed identification of compartment-specific stress reactions. In the symbiont, stress-response a...Continue Reading

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Citations

Feb 9, 2020·Environmental Science and Pollution Research International·Patrícia P B Eichler, Diogo S de Moura
Feb 26, 2021·Journal of Proteome Research·Haiying MaLiang Zhang

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Datasets Mentioned

BETA
PXD008015

Methods Mentioned

BETA
protein folding
thermal
GTPases
thermal stress

Software Mentioned

blastp
Blast2GO
Progenesis LC - MS
R
- HIT suite
SPIDER
CD
PEAKS Studio
Blast
Progenesis

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