Dishevelled interacts with p65 and acts as a repressor of NF-κB-mediated transcription

Cell Research
Ning DengLin Li

Abstract

Dishevelled (Dvl) is a highly conserved protein family that plays an important role in mediating Wnt signaling from membrane to cytoplasm. Recently we reported that Dvl also functions in the nucleus by stabilizing the β-catenin/TCFs transcriptional complex. Here we describe that Dvl may function as a repressor of NF-κB. Our data show that Dvl directly binds to p65 and their interaction occurs in the nucleus. Dvl expression inhibits p65-mediated or TNF-α-stimulated activation of the NF-κB dependent reporter. This action of Dvl, however, is not dependent on Wnt or its downstream effector β-catenin. Chromatin immunoprecipitation assay shows that recruitment of p65 to the promoters of NF-κB target genes is significantly enhanced when expression of Dvl is knocked down. Consistently, the expression level of a subset of NF-κB target genes is also increased after knock-down of Dvl. Moreover, our data suggest that Dvl may relieve the anti-apoptotic effect of NF-κB, thus play a role in promoting apoptosis. Therefore, this work demonstrates a novel function of Dvl in modulating NF-κB-regulated gene transcription.

References

Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·H Y SongD V Goeddel
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Nov 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M TsangD J Sussman
Apr 10, 1997·The New England Journal of Medicine·P J Barnes, M Karin
Apr 1, 1997·Trends in Genetics : TIG·R T MoonM Torres
May 23, 1998·Annual Review of Immunology·S GhoshE B Kopp
Oct 3, 1999·Mechanisms of Development·M Boutros, M Mlodzik
Nov 11, 1999·Oncogene·F Mercurio, A M Manning
Dec 10, 1999·Experimental Cell Research·E T Strovel, D J Sussman
Dec 22, 1999·Oncogene·B Rayet, C Gélinas
Sep 12, 2003·Developmental Cell·Michael T VeemanRandall T Moon
Jun 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·D Scott WitherowRobert J Lefkowitz
Sep 17, 2004·Genes & Development·Matthew S Hayden, Sankar Ghosh
Jan 20, 2005·Molecular and Cellular Biology·Bin LiuKe Shuai
Feb 22, 2005·Journal of Biology·Keiji ItohSergei Y Sokol
Mar 7, 2006·Developmental Cell·Bob GoldsteinHitoshi Sawa
Jul 11, 2007·The Journal of Cell Biology·Shaogang SunChen Wang
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse

❮ Previous
Next ❯

Citations

Nov 21, 2012·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Catriona KellyBettina C Schock
Mar 25, 2020·Critical Reviews in Biochemistry and Molecular Biology·Linxi LiC Yan Cheng
Sep 24, 2020·International Journal of Rheumatic Diseases·Jie LiJin-Qi Li
Jul 26, 2018·International Journal of Molecular Sciences·Yu XunShuanglin Xiang
Jun 13, 2017·Frontiers in Cell and Developmental Biology·Marc Gentzel, Alexandra Schambony
Aug 10, 2020·Journal of Molecular Cell Biology·Qi YangJizheng Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis