Dismantling of Arabidopsis thaliana mesophyll cell chloroplasts during natural leaf senescence.

Plant Biology
I M EvansJ A Brusslan

Abstract

One of the earliest events in the process of leaf senescence is dismantling of chloroplasts. Mesophyll cell chloroplasts from rosette leaves were studied in Arabidopsis thaliana undergoing natural senescence. The number of chloroplasts decreased by only 17% in fully yellow leaves, and chloroplasts were found to undergo progressive photosynthetic and ultrastructural changes as senescence proceeded. In ultrastructural studies, an intact tonoplast could not be visualized, thus, a 35S-GFP::delta-TIP line with a GFP-labeled tonoplast was used to demonstrate that chloroplasts remain outside of the tonoplast even at late stages of senescence. Chloroplast DNA was measured by real-time PCR at four different chloroplast loci, and a fourfold decrease in chloroplast DNA per chloroplast was noted in yellow senescent leaves when compared to green leaves from plants of the same age. Although chloroplast DNA did decrease, the chloroplast/nuclear gene copy ratio was still 31:1 in yellow leaves. Interestingly, mRNA levels for the four loci differed: psbA and ndhB mRNAs remained abundant late into senescence, while rpoC1 and rbcL mRNAs decreased in parallel to chloroplast DNA. Together, these data demonstrate that, during senescence, chloroplasts...Continue Reading

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Citations

Mar 26, 2014·Journal of Experimental Botany·Rubén Casanova-SáezJosé Luis Micol
Mar 29, 2014·The Plant Cell·Hieronim GolczykReinhold G Herrmann
Sep 13, 2014·Journal of Plant Physiology·Wouter G van Doorn, Domenico Prisa
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Dec 17, 2011·Plant, Cell & Environment·Bastiaan BrouwerPer Gardeström
Jun 8, 2012·Plant Biology·L C RibeiroF Borghetti
Oct 23, 2013·Autophagy·Wouter G van Doorn, Alessio Papini
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Mar 18, 2019·Genes & Genomics·Jeongsik Kim
Oct 11, 2017·Journal of Experimental Botany·Marisa S Otegui
Dec 20, 2019·The Plant Journal : for Cell and Molecular Biology·Stephan GreinerReinhold G Herrmann
Apr 23, 2021·The Plant Journal : for Cell and Molecular Biology·Ryuuichi D ItohMakoto T Fujiwara
Jul 6, 2021·Frontiers in Plant Science·Heebak ChoiSun-Hwa Ha

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