Disordered protein-graphene oxide co-assembly and supramolecular biofabrication of functional fluidic devices.

Nature Communications
Yuanhao WuAlvaro Mata

Abstract

Supramolecular chemistry offers an exciting opportunity to assemble materials with molecular precision. However, there remains an unmet need to turn molecular self-assembly into functional materials and devices. Harnessing the inherent properties of both disordered proteins and graphene oxide (GO), we report a disordered protein-GO co-assembling system that through a diffusion-reaction process and disorder-to-order transitions generates hierarchically organized materials that exhibit high stability and access to non-equilibrium on demand. We use experimental approaches and molecular dynamics simulations to describe the underlying molecular mechanism of formation and establish key rules for its design and regulation. Through rapid prototyping techniques, we demonstrate the system's capacity to be controlled with spatio-temporal precision into well-defined capillary-like fluidic microstructures with a high level of biocompatibility and, importantly, the capacity to withstand flow. Our study presents an innovative approach to transform rational supramolecular design into functional engineering with potential widespread use in microfluidic systems and organ-on-a-chip platforms.

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Citations

Apr 11, 2020·Nature Chemistry·Ankit Jain, Rein V Ulijn
Oct 8, 2020·Journal of Tissue Engineering and Regenerative Medicine·Shizhou WuHockin H K Xu
Jan 8, 2021·Biomaterials·Raquel C GonçalvesJoão F Mano
Jul 13, 2021·ACS Biomaterials Science & Engineering·Diego López BarreiroCees M J Sagt
Jul 25, 2021·Colloids and Surfaces. B, Biointerfaces·Laura ChronopoulouCleofe Palocci
Sep 25, 2020·ACS Applied Energy Materials·Debanjan DharMatthew R Lockett
Aug 28, 2020·Biomacromolecules·Miguel González-PérezJosé Carlos Rodríguez-Cabello

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Methods Mentioned

BETA
confocal microscopy
SANS
Small-angle neutron scattering
electrophoresis
infrared spectroscopy
neutron scattering
Assay

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