Dispersal of Epithelium-Associated Pseudomonas aeruginosa Biofilms.

MSphere
Anna C ZemkeJennifer M Bomberger

Abstract

Pseudomonas aeruginosa grows in highly antibiotic-tolerant biofilms during chronic airway infections. Dispersal of bacteria from biofilms may restore antibiotic susceptibility or improve host clearance. We describe models to study biofilm dispersal in the nutritionally complex environment of the human airway. P. aeruginosa was cocultured in the apical surface of airway epithelial cells (AECs) in a perfusion chamber. Dispersal, triggered by sodium nitrite, a nitric oxide (NO) donor, was tracked by live cell microscopy. Next, a static model was developed in which biofilms were grown on polarized AECs without flow. We observed that NO-triggered biofilm dispersal was an energy-dependent process. From the existing literature, NO-mediated biofilm dispersal is regulated by DipA, NbdA, RbdA, and MucR. Interestingly, altered signaling pathways appear to be used in this model, as deletion of these genes failed to block NO-induced biofilm dispersal. Similar results were observed using biofilms grown in an abiotic model on glass with iron-supplemented cell culture medium. In cystic fibrosis, airway mucus contributes to the growth environment, and a wide range of bacterial phenotypes are observed; therefore, we tested biofilm dispersal in a...Continue Reading

References

Jan 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A L CozensD C Gruenert
Jan 25, 2002·Journal of Bacteriology·Karin SauerDavid G Davies
Nov 25, 2003·Nature·Thien-Fah MahGeorge A O'Toole
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hemantha KulasakaraStephen Lory
Mar 7, 2006·Applied and Environmental Microbiology·Ehud BaninE Peter Greenberg
May 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eric E SmithMaynard V Olson
Jul 6, 2006·Applied and Environmental Microbiology·Robert M Q ShanksGeorge A O'Toole
Oct 20, 2006·Journal of Bacteriology·Nicolas BarraudJeremy S Webb
Apr 5, 2007·Nature Protocols·Kyoung-Hee Choi, Herbert P Schweizer
Jan 19, 2008·American Journal of Respiratory and Critical Care Medicine·Timothy F MurphySanjay Sethi
Mar 25, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sophie Moreau-MarquisBruce A Stanton
May 13, 2008·Journal of Bacteriology·Li Zhang, Thien-Fah Mah
Dec 17, 2008·Journal of Bacteriology·David G Davies, Cláudia N H Marques
Jan 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Sophie Moreau-MarquisBruce A Stanton
Oct 26, 2010·Journal of Visualized Experiments : JoVE·Sophie Moreau-MarquisGregory G Anderson
Feb 11, 2011·Journal of Visualized Experiments : JoVE·George A O'Toole
Feb 24, 2011·Antimicrobial Agents and Chemotherapy·Bruce D McCollisterAndrés Vázquez-Torres
Apr 12, 2012·Journal of Bacteriology·Ankita Basu RoyKarin Sauer
Jul 3, 2013·Pediatric Pulmonology·Nicole Mayer-HamblettUNKNOWN ESCF Investigators
Jul 6, 2013·Trends in Microbiology·Thomas BjarnsholtNiels Høiby
Jan 29, 2014·American Journal of Respiratory and Critical Care Medicine·Benjamin J StaudingerPradeep K Singh
May 14, 2014·Methods in Molecular Biology·Nicolas BarraudAlain Filloux
May 20, 2014·Methods in Molecular Biology·Daniel E Deatherage, Jeffrey E Barrick
Mar 25, 2015·Antimicrobial Agents and Chemotherapy·Anna C ZemkeJennifer M Bomberger
May 15, 2015·Frontiers in Microbiology·Azadeh Rahmani-BadiSaeed Heidari-Keshel
Aug 25, 2015·Cell Host & Microbe·Peter JorthPradeep K Singh

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Citations

May 4, 2021·Applied Microbiology and Biotechnology·Yu-Ming CaiLiang Yang

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Methods Mentioned

BETA
scanning light microscopy
transfection
PCR

Software Mentioned

Prism
GraphPad
Breseq
Nikon Elements

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