Dispersed and aggregated gap junction channels identified by immunogold labeling of freeze-fractured membranes

Experimental Cell Research
D F HülserO Traub

Abstract

An indirect immunogold labeling technique was applied to replicas of freeze-fractured membranes of rapidly frozen unfixed cells. The endogenous gap junction protein Cx43 of BICR/M1Rk rat mammary tumor cells was preferentially identified in quasi-crystalline gap junction plaques as were the transfected connexins Cx40, Cx43, and Cx45 in HeLa (human cervical carcinoma) cells. With this method we also detected contact areas with dispersed gap junction channels which are the only structural correlation for endogenous Cx45 in HeLa wild-type cells where no gap junction plaques exist. In double-transfected HeLa cells a colocalization of Cx40 and Cx43 was occasionally detected in quasi-crystalline gap junction plaques, whereas in contact areas with dispersed particles only one Cx type was present. Our results indicate that functional gap junction channels exist outside the quasi-crystalline plaques.

References

Aug 1, 1976·The Journal of Cell Biology·C Peracchia, A F Dulhunty
Jan 1, 1992·Electron Microscopy Reviews·G E Sosinsky
Mar 1, 1987·The Journal of Cell Biology·W T GruijtersD A Goodenough
Sep 1, 1973·Zeitschrift Für Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie·D F Hülser, A Demsey
Jul 1, 1973·Experimental Cell Research·D F Hülser, D J Webb
Oct 1, 1972·European Journal of Immunology·M F Rajewsky, A Grüneisen
Dec 1, 1981·The Journal of Cell Biology·L T Haimo, J L Rosenbaum
Oct 1, 1980·The Journal of Cell Biology·E RaviolaG Raviola
Aug 1, 1993·Pflügers Archiv : European journal of physiology·R EckertD F Hülser
Jan 1, 1995·International Review of Cytology·H Wolburg, A Rohlmann
Aug 1, 1995·Microscopy Research and Technique·R R Shivers, L K McVicar
Aug 1, 1995·Microscopy Research and Technique·J E Hall, R G Gourdie
Feb 9, 1996·Cell·N M Kumar, N B Gilula
Jan 1, 1996·Annual Review of Biochemistry·D A GoodenoughD L Paul
Aug 1, 1996·Journal of Bioenergetics and Biomembranes·D W Laird

❮ Previous
Next ❯

Citations

Apr 22, 2005·Biological Chemistry·Stephanie BreidertHassan Y Naim
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Undine LaufMatthias M Falk
Mar 21, 2012·Biochemical and Biophysical Research Communications·Praween WayakanonIkuo Morita
Dec 26, 2006·Biophysical Journal·Mindaugas RackauskasFeliksas F Bukauskas
Mar 23, 2004·Biochimica Et Biophysica Acta·Dominique Segretain, Matthias M Falk
Jan 29, 2002·Journal of Molecular Biology·Galen M HandGina E Sosinsky
Jun 26, 1999·Circulation Research·S AlcoléaD B Gros
Feb 10, 2000·Circulation Research·V ValiunasP R Brink
Jul 7, 2007·American Journal of Physiology. Cell Physiology·Sabrina W YumSteven S Scherer
Dec 24, 2004·The Journal of Biological Chemistry·Julian ThimmRatnesh Lal
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·V ValiunasE C Beyer
Apr 13, 2000·Brain Research·V M BerthoudJ C Sáez
Sep 23, 2000·European Journal of Cell Biology·E L BenedettiH Bloemendal
Feb 24, 2001·European Journal of Cell Biology·M L Rütz, D F Hülser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.