PMID: 1211449Sep 1, 1975Paper

Dispersed rat parotid acinar cells. III. Characterization of cholinergic receptors

The American Journal of Physiology
J A MangosT Barber

Abstract

The in vitro characterization of cholinergic receptors in dispersed rat parotid acinar cells was accomplished through investigations of the net transmembrane fluxes of K in response to exposure of the cells to selected cholinergic agonists and antagonists. Interaction of acetylcholine bromide (ACh) and carbamylcholine (carbachol) with the cholinergic receptors resulted in rapid net efflux of K from the cells. This cellular response was demonstrable in concentrations of carbachol as low as 10(-8) M. With gradual increase in the concentrations of the agonist an increase in the K efflux was observed up to 10(-5) M. At higher concentrations of this cholinergic agonist no further increases in the net K efflux were observed. The response of the cells to cholinergic agonists was inhibited by atropine but not by the adrenergic antagonists phentolamine or propranolol, suggesting cholinergic agonist-antagonist interactions at the receptor site. The dispersed rat parotid acinar cells appear to have functionally intact cholinergic receptors and could be used as valuable experimental tools for the study of receptor physiology and pharmacology as well as of other aspects of secretory function at the cellular level.

Citations

Aug 1, 1989·Pflügers Archiv : European journal of physiology·M MurakamiH Watari
Feb 1, 1987·Journal of Dental Research·K T IzutsuM Goddard
Mar 1, 1980·Journal of Morphology·N FlemingJ M Sturgess
Jan 1, 1981·Journal of Dental Research·J A Mangos, W H Donnelly
Jan 1, 1982·Pflügers Archiv : European journal of physiology·J Poggioli, J W Putney
Oct 1, 1977·Archives internationales de physiologie et de biochimie·G Dandrifosse
Aug 1, 1980·Journal of Dental Research·J A MangosW Farnham
May 1, 1995·Clinical and Experimental Pharmacology & Physiology·B PeterA W Konings
May 1, 1979·Journal of Dental Research·J A Mangos
Jun 27, 1978·Archives of Oto-rhino-laryngology·R Chilla, C Arglebe
May 1, 1995·Archives of Oral Biology·J C BrooksJ D Watson
Jan 1, 1988·Archives of Oral Biology·K IzutsuE Truelove
Jun 1, 1977·Kidney International·A D Macknight

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