Dispersive micro-solid-phase extraction combined with online preconcentration by capillary electrophoresis for the determination of glycopyrrolate stereoisomers in rat plasma

Journal of Separation Science
Yongjing LiuDawei Chen

Abstract

A simple and sensitive analytical method for four isomers of glycopyrrolate in rat plasma was developed using cation-selective exhaustive injection-sweeping cyclodextrin-modified electrokinetic chromatography (CSEI-Sweeping-CDEKC) for online enrichment combined with dispersive micro-solid-phase extraction pretreatment. The CSEI-Sweeping-CDEKC was conducted on an uncoated fused silica capillary (40.2 cm × 75 μm) with an applied voltage of -20 kV. The electrophoretic analysis was carried out in 30 mM phosphate solution at pH 2.0 containing 20 mg/mL sulfated-β-cyclodextrin and 5% acetonitrile. Under these optimized conditions, the detection limit for racemic glycopyrrolate was found to be 2.0 ng/mL and this method could increase 495-fold detection sensitivity compared with the traditional injection method. Additionally, the parameters that affected the extraction efficiency of dispersive micro-solid-phase extraction were also examined systematically. The glycopyrrolate isomers in rat plasma samples as low as 0.0625 μg/mL were able to be separated and detected by capillary electrophoresis with the aid of CSEI-sweeping. The findings of this study show that the dispersive micro-solid-phase extraction pretreatment coupled with CSEI-Sw...Continue Reading

References

Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mei-Chuan ChenCheng-Huang Lin
Dec 29, 2007·Electrophoresis·Laura Sánchez-HernándezCarmen García-Ruiz
Apr 22, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jun WenGuo-Rong Fan
May 24, 2014·Electrophoresis·Pavel RezankaDavid Sýkora
Sep 7, 2017·Journal of Analytical Toxicology·Shaohua Li, Dawei Chen

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