Dispersive solid-phase extraction for the determination of sulfonamides in chicken muscle by liquid chromatography

Journal of Chromatography. a
Andrzej PosyniakK Mitrowska

Abstract

A new, fast and low-cost sample preparation for the determination of sulfonamide (SA) residues in chicken muscle by LC technique has been developed. The procedure involves single extraction of sample with acetonitrile, followed by a rapid clean-up and was called "dispersive solid-phase extraction" (dispersive SPE). Using dispersive SPE 25 mg of octadecyl sorbent was added to 1 ml of acetonitrile extract, mixed and centrifuged. The acetonitrile layer was evaporated and residue was dissolved in acetate buffer (pH 3.5). Analysed compounds were detected by fluorescence detector after pre-column derivatization with fluorescamine. The separation of analytes was performed with gradient elution with mobile phase methanol: 2% acetic acid and RP-LC analytical column. The whole procedure was evaluated for six sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine, sulfametoxypirydazine, sulfametoxazole and sulfadimetoxine) according to the European Commission Decision 2002/657/EC. Specificity, decision limit (CCalpha), detection capacity (CCbeta), trueness and precision were determined during validation process. The dispersive SPE with octadecyl sorbent was found suitable for sample preparation before sulfonamide determination in chick...Continue Reading

Citations

May 21, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiao-Lin HouXiang-Dang Du
Apr 16, 2011·Journal of Agricultural and Food Chemistry·Sílvia BorràsJacinto Guiteras
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Dec 31, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mahmoud A OmarSayed M Derayea
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Nov 29, 2017·International Journal of Analytical Chemistry·Gabriela IslasAlberto Cepeda

Related Concepts

Metazoa
Liquid Chromatography
Muscle
Standardization
Statistical Sensitivity
Fluorescence Spectroscopy
Sulfonamides
Reproducibility of Results

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