Displaced starburst amacrine cells of the rabbit retina contain the 67-kDa isoform, but not the 65-kDa isoform, of glutamate decarboxylase

Visual Neuroscience
C Brandon, M H Criswell

Abstract

The cholinergic identity of retinal starburst amacrine neurons is well established, but recent evidence suggests that these cells are GABAergic as well. Confirmation of this dual transmitter function requires the demonstration of glutamate decarboxylase (GAD), the biosynthetic enzyme for GABA, within starburst cells. The current work was undertaken to determine whether rabbit retinal starburst amacrine neurons contain either of the two known isoforms of GAD. To do this, we have examined the localization of the following: (1) the 65-kDa isoform of GAD; (2) the 67-kDa isoform of GAD; (3) choline acetyltransferase; and (4) the fluorescent dye DAPI, a marker for cholinergic amacrine cells. In addition, we labeled displaced starburst neurons directly, by injecting them with Lucifer Yellow in vitro. Four strata within the inner plexiform layer contained immunoreactive GAD65. A non-GAD65-immunoreactive zone separated the two innermost strata (G3 and G4); this zone contained (1) the dendrites of individual Lucifer Yellow-injected, displaced starburst amacrine cells; (2) dendrites immunoreactive for choline acetyltransferase; and (3) processes of DAPI-labeled amacrine cells. Immunoreactive GAD67 appeared in the same strata that containe...Continue Reading

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Citations

Jul 20, 2005·Journal of Neurochemistry·Lee StewartStefano Stifani
Aug 20, 2003·The Journal of Comparative Neurology·Daniel SunMichael Kalloniatis
Sep 24, 2005·Journal of Integrative Neuroscience·Roman R Poznanski
Jul 27, 2011·Visual Neuroscience·Kevin J Ford, Marla B Feller
Aug 24, 2018·Scientific Reports·Hélène LégerFrancis C Luca
May 11, 2000·The Journal of Comparative Neurology·L T Nguyen, N M Grzywacz
Mar 15, 2001·Neurochemistry International·D V Pow

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