Displacement immunoassay for the detection of ochratoxin A using ochratoxin B modified glass beads

Analytical and Bioanalytical Chemistry
Vasilica LatesJean-Louis Marty

Abstract

We report here the development of a new assay for the detection of ochratoxin A (OTA) based on the use of its dechlorinated analogue, ochratoxin B (OTB), in a displacement immunoassay. OTB was immobilised on controlled-pore glass beads followed by the binding of anti-OTA antibody, with anti-IgG antibody peroxidase conjugate used as a label. In this manner, an original bio-sensing material was obtained. Upon incubation of this material with OTA, the toxin competes with OTB for the binding sites of the anti-OTA antibodies and releases the antibody-tagged peroxidase complex into the solution. Compared to classic competitive immunoassays, this newly developed displacement immunoassay presents a similar detection limit and assay time. Moreover, the detection, based on the activity of the horseradish peroxidase, is performed for the first time in situ using wine samples.

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Citations

Mar 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zheng HanAibo Wu
Nov 8, 2013·Toxins·Amina RhouatiJean-Louis Marty
Dec 23, 2015·Toxins·Tai Hwan Ha
Apr 12, 2018·Sensors·Şerife Şeyda PirinçciSelma Öztürk

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