Display of membrane proteins on the heterologous caveolae carved by caveolin-1 in the Escherichia coli cytoplasm

Enzyme and Microbial Technology
Jonghyeok ShinDae-Hyuk Kweon

Abstract

Caveolae are membrane-budding structures that exist in many vertebrate cells. One of the important functions of caveolae is to form membrane curvature and endocytic vesicles. Recently, it was shown that caveolae-like structures were formed in Escherichia coli through the expression of caveolin-1. This interesting structure seems to be versatile for a variety of biotechnological applications. Targeting of heterologous proteins in the caveolae-like structure should be the first question to be addressed for this purpose. Here we show that membrane proteins co-expressed with caveolin-1 are embedded into the heterologous caveolae (h-caveolae), the cavaolae-like structures formed inside the cell. Two transmembrane SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, Syntaxin 1a and vesicle-associated membrane protein 2 (VAMP2), were displayed on the h-caveolae surface. The size of the h-caveolae harboring the transmembrane proteins was ∼100 nm in diameter. The proteins were functional and faced outward on the h-caveolae. Multi-spanning transmembrane proteins FtsH and FeoB could be included in the h-caveolae, too. Furthermore, the recombinant E. coli cells were shown to endocytose substrate supplemen...Continue Reading

References

Sep 15, 1975·FEBS Letters·G EytanE Racker
Nov 1, 1973·Biochemical and Biophysical Research Communications·E Racker
May 30, 1974·Biochimica Et Biophysica Acta·D PapahadjopoulosW J Vail
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S EmtageP A Lowe
Oct 6, 1995·The Journal of Biological Chemistry·Y AkiyamaK Ito
Apr 7, 1998·Cell·T WeberJ E Rothman
Nov 18, 1998·Biochemistry·J KnolB Poolman
Oct 6, 1999·Current Opinion in Biotechnology·F Baneyx
Jun 5, 2003·Trends in Microbiology·Klaus Hantke
Sep 14, 2004·Current Opinion in Lipidology·Philippe G Frank, Michael P Lisanti
Oct 14, 2004·Applied Microbiology and Biotechnology·F R Schmidt
Mar 15, 2005·Biotechnology Advances·F J M MergulhãoG A Monteiro
Aug 30, 2005·Biochimica Et Biophysica Acta·Lucas Pelkmans
Dec 22, 2005·Nature Methods·Niraj H Tolia, Leemor Joshua-Tor
Sep 26, 2006·Protein Expression and Purification·Davide RoncaratiVincenzo Scarlato
Dec 2, 2006·Nature Reviews. Drug Discovery·John P OveringtonAndrew L Hopkins
Mar 6, 2010·Recent Patents on Biotechnology·Sung Ho YoonJihyun F Kim
Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yoosoo YangDae-Hyuk Kweon
Aug 7, 2012·Applied and Environmental Microbiology·Guo-Shu WangRobin Ghosh
Aug 21, 2012·Cell·Piers J WalserRobert G Parton
Jan 25, 2013·International Journal of Molecular Sciences·Hsin-Hui ShenLisa Martin
Mar 14, 2013·FEMS Microbiology Reviews·Marizela DelicBrigitte Gasser

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Citations

May 26, 2021·Advanced Science·Dongsoo YangSang Yup Lee
Apr 25, 2019·ACS Synthetic Biology·Jonghyeok ShinDae-Hyuk Kweon

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